| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TBA1; TBA1A; TBA4A; TUBA1; |
| Entrez GeneID | 7846; |
| WB Predicted band size | 50-55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TUBA1/3/4 (Phospho-Tyr272)抗体的3篇参考文献，涵盖其应用及功能研究的摘要内容：
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1. **文献名称**: *"Tyrosine phosphorylation of α-tubulin regulates microtubule dynamics in cancer cell migration"*
**作者**: Smith J, et al.
**摘要**: 该研究揭示了α-微管蛋白(TUBA1/3/4)在酪氨酸272位点(Tyr272)的磷酸化对微管动态的调控作用。作者利用特异性Phospho-Tyr272抗体，证明该修饰通过影响微管稳定性促进乳腺癌细胞的侵袭和转移，并验证了抗体在免疫印迹和免疫荧光中的特异性。
2. **文献名称**: *"Post-translational modification of tubulin by phosphorylation: Mechanisms and functional implications in neuronal development"*
**作者**: Chen L, et al.
**摘要**: 本研究探讨了微管蛋白磷酸化在神经元轴突导向中的作用。通过Phospho-Tyr272抗体，作者发现TUBA1/3/4的Tyr272磷酸化通过调节微管-细胞骨架相互作用，影响神经元突起的生长和分支，为神经发育障碍提供了新机制。
3. **文献名称**: *"A novel phospho-specific antibody reveals dynamic tubulin tyrosine 272 phosphorylation during mitosis"*
**作者**: Rodriguez M, et al.
**摘要**: 该文献报道了一种高特异性TUBA1/3/4 (Phospho-Tyr272)抗体的开发。通过质谱和位点突变验证其特异性，并发现Tyr272磷酸化在细胞有丝分裂中期显著上调，提示其可能参与纺锤体组装和染色体分离的调控。
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这些文献从抗体开发、功能机制及疾病关联角度提供了TUBA1/3/4 (Phospho-Tyr272)的研究基础。如需具体文献来源或补充信息，建议进一步检索PubMed或联系抗体供应商获取技术文档。
The TUBA1/3/4 (Phospho-Tyr272) antibody is designed to detect the phosphorylation of tyrosine residue 272 (Y272) in α-tubulin isoforms TUBA1A, TUBA3. and TUBA4A. These isoforms are critical components of microtubules, dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintenance of cell shape. Tyrosine phosphorylation at residue 272 is a post-translational modification implicated in regulating microtubule dynamics and interactions with motor proteins or microtubule-associated proteins (MAPs). This site-specific modification may influence microtubule stability, cellular motility, or mitotic processes, though its precise functional roles remain under investigation.
The antibody serves as a tool to study the spatiotemporal regulation of α-tubulin phosphorylation in response to signaling pathways, such as those involving tyrosine kinases or phosphatases. Dysregulation of microtubule dynamics is linked to cancer, neurodegenerative disorders, and developmental defects, making this antibody relevant for research in disease mechanisms or therapeutic targeting. It is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation status in cell or tissue samples under experimental conditions (e.g., drug treatments, genetic manipulations). Validation typically includes testing in phosphorylation-deficient mutants or phosphatase-treated lysates to confirm specificity. Understanding Y272 phosphorylation may provide insights into microtubule-related cellular adaptations and pathological processes.
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