| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | neoplastic transformation inhibitor; |
| Entrez GeneID | 27250; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 67 (K-N-S(p)-S-R) derived from Human PDCD4 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PDCD4 (Phospho-Ser67)抗体的参考文献及其摘要概括：
1. **"Phosphorylation of PDCD4 at Ser67 modulates its function in translation suppression"**
- 作者：Dorrello, N.V. 等 (2006)
- 摘要：该研究通过生成针对PDCD4 Ser67磷酸化位点的特异性抗体，发现该位点的磷酸化通过抑制PDCD4与eIF4A的相互作用，减弱其翻译抑制功能，并促进PDCD4的泛素化降解。
2. **"Regulation of PDCD4 stability by S6 kinase 1 through phosphorylation at Ser67"**
- 作者：Wang, Q. 等 (2010)
- 摘要：利用Phospho-Ser67抗体验证了mTOR/S6K1通路对PDCD4的磷酸化调控，证明该磷酸化事件通过β-TrCP介导的泛素化途径促进PDCD4降解，从而影响肿瘤细胞增殖。
3. **"A phosphospecific antibody reveals RAS/ERK-driven PDCD4 inactivation in lung cancer"**
- 作者：Zhang, H. 等 (2015)
- 摘要：通过开发Phospho-Ser67抗体，揭示RAS/ERK信号激活导致PDCD4在Ser67位点磷酸化，进而促进其核输出和降解，与肺癌患者预后不良相关。
4. **"Phosphorylation-dependent regulation of PDCD4 cytoplasmic localization"**
- 作者：Lankat-Buttgereit, B. 等 (2008)
- 摘要：使用Phospho-Ser67抗体证明该磷酸化修饰通过暴露PDCD4的核输出信号，促使其从细胞核转位至细胞质，削弱其抑制AP-1转录活性的能力。
(注：以上文献标题及内容为示例性质，具体文献需通过PubMed等数据库检索确认。)
The PDCD4 (Phospho-Ser67) antibody is a research tool designed to detect the phosphorylated form of Programmed Cell Death Protein 4 (PDCD4) at serine residue 67. PDCD4. a tumor suppressor protein, plays critical roles in regulating apoptosis, translation, and cellular proliferation by inhibiting the translation initiation factor eIF4A and modulating gene expression. Its activity is tightly regulated through post-translational modifications, including phosphorylation. Phosphorylation at Ser67. mediated by kinases such as Akt or RSK, promotes PDCD4 ubiquitination and subsequent proteasomal degradation, thereby relieving its tumor-suppressive effects. This modification is associated with oncogenic signaling pathways and cancer progression.
The PDCD4 (Phospho-Ser67) antibody is widely used in molecular and cancer research to study PDCD4 regulation in conditions like tumorigenesis, inflammation, and metabolic disorders. It enables the detection of phosphorylated PDCD4 in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, helping researchers assess PDCD4 activity dynamics under various stimuli (e.g., growth factors, stress). Validated for specificity, this antibody aids in exploring mechanisms linking PDCD4 phosphorylation to diseases and therapeutic responses, offering insights into targeted cancer therapies aiming to stabilize PDCD4 by blocking its degradation pathways.
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