| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SRBP1; SREBF1; SREBP1; sterol regulatory element-binding protein 1; |
| Entrez GeneID | 6720; |
| WB Predicted band size | 122kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine439(Q-S-S(p)-P-L) derived from Human SREBP-1 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SREBP-1 (Phospho-Ser439)抗体的3篇参考文献示例(注：文献信息为模拟概括，具体内容需通过学术数据库验证)：
1. **文献名称**：*"Regulation of SREBP-1 phosphorylation by insulin signaling"*
**作者**：Shimano H, et al.
**摘要**：研究揭示了胰岛素通过AKT/mTOR通路诱导SREBP-1在Ser439位点的磷酸化，促进其成熟及脂质合成基因的激活，抗体用于检测该修饰在肝脏代谢中的作用。
2. **文献名称**：*"Phosphorylation of SREBP-1 at Ser439 modulates hepatic lipid accumulation in obesity"*
**作者**：Li X, et al.
**摘要**：通过高脂饮食小鼠模型，利用Phospho-Ser439抗体证实SREBP-1磷酸化水平与脂肪肝进展相关，提示其作为代谢疾病治疗靶点的潜力。
3. **文献名称**：*"A monoclonal antibody specific to SREBP-1 phosphorylated at Ser439 reveals its role in cancer cell proliferation"*
**作者**：Wang Y, et al.
**摘要**：开发了针对Ser439磷酸化位点的单克隆抗体，发现肿瘤细胞中该修饰通过上调脂代谢促进细胞增殖，抗体被用于体外及小鼠模型中的机制验证。
**建议**：以上文献信息为示例，实际引用时请通过PubMed或Google Scholar搜索准确文献，使用关键词“SREBP-1 Phospho-Ser439 antibody”或“SREBP-1 Ser439 phosphorylation”进行验证。
**Background of SREBP-1 (Phospho-Ser439) Antibody**
Sterol regulatory element-binding protein 1 (SREBP-1) is a key transcription factor regulating lipid metabolism, particularly fatty acid and cholesterol synthesis. It exists as two isoforms, SREBP-1a and SREBP-1c, which are synthesized as inactive precursors bound to the endoplasmic reticulum (ER). Upon sterol depletion, SREBP-1 undergoes proteolytic cleavage, releasing its active N-terminal domain to translocate into the nucleus and activate target genes.
Phosphorylation plays a critical role in modulating SREBP-1 activity. The SREBP-1 (Phospho-Ser439) antibody specifically detects SREBP-1 phosphorylated at serine residue 439. a post-translational modification linked to regulatory mechanisms influencing its stability, nuclear localization, or transcriptional activity. This phosphorylation event may be mediated by kinases such as AKT or mTORC1. connecting SREBP-1 to insulin signaling and nutrient-sensing pathways.
Research using this antibody focuses on metabolic disorders (e.g., obesity, diabetes, fatty liver disease) and cancer, where dysregulated lipid synthesis is implicated. It is widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study SREBP-1 activation under varying metabolic conditions or therapeutic interventions. Proper validation, including phosphatase treatment or mutant controls, ensures specificity in distinguishing phosphorylated SREBP-1 from its unmodified form. Understanding Ser439 phosphorylation provides insights into metabolic homeostasis and disease mechanisms.
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