| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JAK-2; JAK2; Janus kinase 2; kinase Jak2; |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 931 (M-E-Y(p)-L-P) derived from Human JAK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JAK2 (Phospho-Tyr931)抗体的3篇参考文献的简要总结：
1. **文献名称**: "JAK2 Phosphorylation at Tyrosine 931 Regulates STAT Signaling"
**作者**: Smith et al.
**摘要**: 研究揭示了JAK2在Tyr931位点的磷酸化对STAT蛋白活化的调控作用，通过该抗体验证了其在细胞因子信号传导中的关键功能，并探讨了其与骨髓增殖性疾病的关联。
2. **文献名称**: "Role of JAK2 Tyrosine 931 Phosphorylation in Hematopoietic Progenitor Cells"
**作者**: Lee & Kim
**摘要**: 利用Phospho-Tyr931抗体发现，JAK2在此位点的磷酸化影响造血干细胞的增殖分化，并证实其在白血病模型中异常激活的机制。
3. **文献名称**: "JAK2 Y931 Phosphorylation as a Biomarker in Myeloproliferative Neoplasms"
**作者**: Garcia et al.
**摘要**: 通过该抗体的免疫印迹分析，提出JAK2 Tyr931磷酸化水平可作为骨髓增殖性肿瘤的诊断标志物，并验证了其与患者预后的相关性。
(注：上述文献为示例，实际研究中该位点可能较少被报道，建议通过PubMed或抗体供应商的技术文档获取具体文献。)
The JAK2 (Phospho-Tyr931) antibody is a critical tool for studying the activation and regulation of Janus kinase 2 (JAK2), a non-receptor tyrosine kinase central to cytokine receptor signaling. JAK2 mediates intracellular signaling pathways, including JAK-STAT, MAPK, and PI3K/AKT, by phosphorylating downstream targets upon cytokine or growth factor binding. Phosphorylation at tyrosine 931 (Y931) is a key post-translational modification associated with JAK2 activation. This site lies within the kinase domain, and its phosphorylation is thought to regulate enzymatic activity, substrate interactions, or autoinhibition release. Dysregulated JAK2 signaling, particularly hyperactivation due to mutations like V617F, is linked to myeloproliferative neoplasms, hematologic malignancies, and inflammatory diseases.
The JAK2 (Phospho-Tyr931) antibody specifically detects JAK2 phosphorylated at Y931. enabling researchers to investigate activation dynamics in cell lines, tissues, or primary samples. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess JAK2 activation status under physiological or pathological conditions, including drug treatment or disease models. Validation of this antibody typically includes testing in knockout cells or with phosphatase treatment to confirm phospho-specificity. Its applications extend to studying JAK2-related therapeutic targets, such as inhibitors in clinical trials, and exploring mechanisms of resistance in JAK2-driven disorders. Proper controls are essential to distinguish signal specificity from cross-reactivity with related phosphorylated kinases.
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