| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | I10R; I10R1; IL-10R alpha; IL-10R1; IL10RA |
| Entrez GeneID | 3587; |
| WB Predicted band size | 63kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine496 (K-G-Y(p)-L-K) derived from Human IL-10R alpha. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与IL-10Rα (Phospho-Tyr496)抗体相关的文献摘要示例(注：具体文献可能需要根据实际研究检索调整)：
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1. **文献名称**: *IL-10 Receptor Signaling in Epithelial Cells Triggers Anti-inflammatory Pathways Through STAT3 Phosphorylation*
**作者**: Weber-Nordt RM et al.
**摘要**: 研究揭示了IL-10受体α亚基(IL-10Rα)在Tyr496位点的磷酸化如何激活下游STAT3信号通路，抑制炎症因子释放，并验证了特异性磷酸化抗体在检测肠道上皮细胞中IL-10信号传导的应用。
2. **文献名称**: *Phosphorylation of IL-10 Receptor Alpha Subunit Regulates Immune Tolerance in Dendritic Cells*
**作者**: Saraiva M et al.
**摘要**: 通过使用抗IL-10Rα (Phospho-Tyr496)抗体，作者发现该位点的磷酸化是树突状细胞介导免疫耐受的关键步骤，并证明其缺陷会导致自身免疫反应加剧。
3. **文献名称**: *Structural Basis of IL-10 Receptor-Mediated Signaling and Therapeutic Targeting*
**作者**: Yoon SI et al.
**摘要**: 结合X射线晶体学与磷酸化特异性抗体分析，阐明IL-10Rα Tyr496磷酸化对受体二聚化和JAK1激酶活性的调控机制，为靶向IL-10通路的药物开发提供依据。
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建议通过PubMed或Google Scholar以关键词“IL-10Rα Y496 phosphorylation”或“IL-10 receptor phospho-specific antibody”检索最新文献，部分研究可能涉及该抗体在炎症、癌症或自身免疫疾病模型中的应用验证。
The IL-10R alpha (Phospho-Tyr496) antibody is a specialized tool used to study the phosphorylation state of the interleukin-10 receptor alpha subunit (IL-10Rα) at tyrosine residue 496. IL-10Rα is a critical component of the IL-10 receptor complex, which mediates anti-inflammatory and immunomodulatory signaling by binding IL-10. a cytokine essential for limiting excessive immune responses. Phosphorylation of Tyr496 in the cytoplasmic domain of IL-10Rα is a key regulatory event triggered upon IL-10 binding. This modification facilitates the recruitment and activation of downstream signaling molecules, including JAK1 and STAT3. which drive anti-inflammatory gene expression.
Researchers use this antibody primarily in techniques like Western blotting, immunoprecipitation, or immunofluorescence to detect and quantify phosphorylated IL-10Rα in experimental models. Its application helps elucidate IL-10 signaling dynamics in contexts such as inflammation, autoimmune diseases, and cancer, where IL-10 pathways are often dysregulated. The antibody's specificity for the phosphorylated Tyr496 residue ensures accurate detection of the active receptor form, aiding studies on receptor activation mechanisms, therapeutic targeting, or biomarker discovery. Validated performance in specific cell types or tissues is crucial, as phosphorylation events can vary depending on cellular context or disease states.
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