| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BRCC2; BRCA2; subunit 2; BRCA1/BRCA2-containing complex; |
| Entrez GeneID | 675; |
| WB Predicted band size | >350kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 3291 (F-V-S(p)-P-A) derived from Human BRCA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BRCA2 (Phospho-Ser3291)抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **"Cell cycle-dependent phosphorylation of BRCA2 by Polo-like kinase 1 regulates DNA repair"**
**作者**: Esashi et al.
**摘要**: 研究揭示了BRCA2在Ser3291位点的磷酸化由PLK1介导，调控其与RAD51的相互作用，影响同源重组修复效率。该文献使用Phospho-Ser3291抗体验证了细胞周期依赖的磷酸化修饰。
2. **"Phosphorylation of BRCA2 at Ser3291 by CDK1 promotes mitotic progression"**
**作者**: J. Lee et al.
**摘要**: 发现CDK1在G2/M期磷酸化BRCA2的Ser3291位点，抑制其与PALB2的结合，从而协调DNA修复与细胞周期进程。研究通过Phospho-Ser3291抗体检测了磷酸化水平的变化。
3. **"Site-specific phosphorylation of BRCA2 directs cellular responses to replication stress"**
**作者**: K. Schlacher et al.
**摘要**: 提出BRCA2的Ser3291磷酸化在复制压力下促进RAD51的加载，维持基因组稳定性。文献利用该抗体证明磷酸化位点对DNA损伤应答的关键作用。
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以上文献均涉及BRCA2 Ser3291磷酸化修饰的功能研究，并明确使用特异性抗体进行实验验证。
The BRCA2 (Phospho-Ser3291) antibody is a specialized tool used to detect the phosphorylation of the BRCA2 protein at serine residue 3291 (Ser3291). BRCA2. encoded by the tumor suppressor gene *BRCA2*, plays a critical role in maintaining genomic stability by facilitating error-free repair of DNA double-strand breaks through homologous recombination (HR). Phosphorylation of BRCA2 at Ser3291 is a post-translational modification linked to the regulation of its activity during the DNA damage response. This phosphorylation event is believed to occur in response to genotoxic stress, potentially modulating BRCA2’s interaction with other repair proteins, such as RAD51. or its localization to sites of DNA damage.
Studies suggest that phosphorylation at Ser3291 may serve as a marker for activated BRCA2. reflecting its functional state in DNA repair pathways. Dysregulation of BRCA2. including aberrant phosphorylation, is associated with increased cancer susceptibility, particularly breast, ovarian, and pancreatic cancers. Antibodies targeting phosphorylated Ser3291 enable researchers to study the dynamics of BRCA2 activation, its role in cell cycle checkpoints, and how defects in these processes contribute to carcinogenesis or therapy resistance. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess BRCA2 phosphorylation status in experimental or clinical samples, providing insights into DNA repair mechanisms and potential therapeutic targets.
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