| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADHD7; NTPH; TPH2; |
| Entrez GeneID | 121278; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 19(G-F-S(p)-L-D) derived from Human TPH2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TPH2 (Phospho-Ser19)抗体的参考文献示例(部分为虚构，仅供格式参考)：
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1. **文献名称**：*Phosphorylation of Tryptophan Hydroxylase 2 at Ser19 Modulates Serotonin Biosynthesis*
**作者**：Smith, J. et al.
**摘要**：本研究通过Western blot和免疫组化技术，利用TPH2 (Phospho-Ser19)抗体，揭示了小鼠中缝背核TPH2在Ser19位点的磷酸化对5-羟色胺合成的调控作用，并证明其通过激活钙调蛋白依赖性激酶增强酶活性。
2. **文献名称**：*Stress-Induced Alterations in TPH2 Phosphorylation and Emotional Behavior*
**作者**：Zhang, L. et al.
**摘要**：在慢性应激小鼠模型中，使用TPH2 (Phospho-Ser19)抗体检测到中脑TPH2磷酸化水平显著下降，与焦虑样行为相关，提示该位点的磷酸化可能作为情绪障碍的生物标志物。
3. **文献名称**：*Kinase-Specific Regulation of TPH2 via Ser19 Phosphorylation*
**作者**：Johnson, R. et al.
**摘要**：通过体外激酶实验和抗体特异性验证，研究证实p38 MAPK直接磷酸化TPH2的Ser19位点，并利用该抗体证明磷酸化状态影响神经元中5-HT的释放效率。
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**注**：以上文献为示例性质，具体研究需根据实际发表的论文调整。建议通过PubMed或Google Scholar以关键词“TPH2 Ser19 phosphorylation antibody”检索最新文献。
The TPH2 (Phospho-Ser19) antibody is a specialized tool used to study the phosphorylation status of tryptophan hydroxylase 2 (TPH2), a rate-limiting enzyme in serotonin (5-HT) biosynthesis. TPH2. predominantly expressed in the brainstem's serotonergic neurons (e.g., raphe nuclei), catalyzes the conversion of tryptophan to 5-hydroxytryptophan, a precursor to serotonin. Phosphorylation at serine 19 (Ser19) is a key post-translational modification regulating TPH2 activity, influencing serotonin synthesis and release. This modification is linked to cellular signaling pathways, including Ca²⁺/calmodulin-dependent kinase II (CaMKII), which may modulate enzyme function under physiological or stress conditions.
The Phospho-Ser19 antibody specifically detects TPH2 phosphorylated at Ser19. enabling researchers to investigate serotonin-related neuroadaptations in mood disorders (e.g., depression, anxiety), stress responses, or neurodegenerative diseases. It is validated for techniques like Western blotting, immunohistochemistry, and ELISA, providing insights into TPH2 regulatory mechanisms in both preclinical models and human tissues. Proper controls (e.g., non-phosphorylated TPH2 or knockout samples) are essential to confirm specificity, as cross-reactivity with homologous residues (e.g., TPH1 in peripheral tissues) must be ruled out. This antibody serves as a critical reagent for unraveling serotonin dysregulation in neuropsychiatric research.
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