关键信息
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基因名
bipD
- 应用
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别名
bipD；Translocator Protein BipD
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q3JL26
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表达区间
1-310aa
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氨基酸序列
MNMHVDMGRALTVRDWPALEALAKTMPADAGARAMTDDDLRAAGVDRRVPEQKLGAAIDEFASLRLPDRIDGRFVDGRRANLTVFDDARVAVRGHARAQRNLLERLETELLGGTLDTAGDEGGIQPDPILQGLVDVIGQGKSDIDAYATIVEGLTKYFQSVADVMSKLQDYISAKDDKNMKIDGGKIKALIQQVIDHLPTMQLPKGADIARWRKELGDAVSISDSGVVTINPDKLIKMRDSLPPDGTVWDTARYQAWNTAFSGQKDNIQNDVQTLVEKYSHQNSNFDNLVKVLSGAISTLTDTAKSYLQI
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分子量
41.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
BipD is a key protein involved in the pathogenesis of various bacterial species, particularly in the context of host-pathogen interactions. It is a type III secretion system (T3SS) effector protein that plays a crucial role in subverting host cell functions to promote bacterial survival and proliferation. Research has shown that BipD is essential for the invasion of epithelial tissues, enabling bacteria to manipulate host cellular processes such as cytoskeleton dynamics and immune response. Its significance is underscored by studies highlighting the intricate mechanisms through which BipD modulates signaling pathways and facilitates bacterial entry into host cells. Given the increasing global prevalence of antibiotic-resistant infections, understanding the molecular mechanisms of BipD has become vital for developing new therapeutic strategies. By unraveling the structure-function relationship and interactions of BipD with host cell components, researchers aim to identify potential targets for drug development. Additionally, the recombinant production of BipD provides a valuable tool for biochemical and structural studies, allowing scientists to investigate its functional properties in detail. This background underscores the importance of BipD in bacterial pathogenesis and the necessity for continued research into its role and therapeutic potential.
