| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCF1; P47 phox; NCF-47K; |
| Entrez GeneID | 653361; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为关于 p47 phox (Phospho-Ser345) 抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*"Phosphorylation of p47phox directs NADPH oxidase priming and activation in response to inflammatory stimuli"*
**作者**：El-Benna J. et al.
**摘要**：研究揭示了p47 phox的Ser345磷酸化在NADPH氧化酶激活中的关键作用，通过PKC依赖途径促进ROS生成，并验证了Phospho-Ser345抗体在检测巨噬细胞氧化爆发中的应用。
2. **文献名称**：*"Role of p47phox Ser345 phosphorylation in LPS-induced NADPH oxidase activation"*
**作者**：Dang PM. et al.
**摘要**：利用Phospho-Ser345特异性抗体，证实LPS通过TLR4信号诱导p47 phox的Ser345磷酸化，促进其与细胞膜结合及ROS产生，参与炎症反应调控。
3. **文献名称**：*"Regulation of NADPH oxidase activity by phosphorylation of p47phox at Ser345 in vascular smooth muscle cells"*
**作者**：Lee CF. et al.
**摘要**：研究通过免疫印迹(使用Phospho-Ser345抗体)和功能实验，证明血管紧张素II通过ERK1/2通路磷酸化p47 phox Ser345.增强血管平滑肌NADPH氧化酶活性，促进氧化应激。
注：以上文献信息为示例，实际引用需核对真实文献来源。
The p47 phox (Phospho-Ser345) antibody detects the phosphorylated form of the p47 phox protein at serine residue 345. a key regulatory event in NADPH oxidase activation. p47 phox, encoded by the *NCF1* gene, is a cytosolic subunit of the NADPH oxidase complex, which plays a central role in generating reactive oxygen species (ROS) critical for antimicrobial defense and cellular signaling. In resting cells, p47 phox remains autoinhibited through intramolecular interactions. Upon stimulation (e.g., by cytokines, pathogens, or growth factors), phosphorylation at multiple serine residues, including Ser345. induces conformational changes that unmask binding sites, enabling p47 phox to translocate to the membrane and assemble with other subunits (e.g., gp91 phox, p22 phox) to form the active oxidase.
Phosphorylation at Ser345 is mediated by kinases such as protein kinase C (PKC) or mitogen-activated protein kinases (MAPKs) and is associated with NADPH oxidase activation in neutrophils, macrophages, and other phagocytic cells. The p47 phox (Phospho-Ser345) antibody is widely used in immunological research to study oxidative burst mechanisms, inflammatory responses, and diseases linked to ROS dysregulation, such as chronic granulomatous disease (CGD), atherosclerosis, and autoimmune disorders. Its specificity for the phosphorylated form allows researchers to assess activation status of the NADPH oxidase pathway in experimental models, including Western blotting, immunofluorescence, and flow cytometry. Validation typically involves testing in knockout or phosphorylation-deficient cell lines to confirm signal specificity.
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