| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IER3; NFBD1; KIAA0170; DKFZp781A0122; |
| Entrez GeneID | 9656; |
| WB Predicted band size | 226kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **MDC1 (Phospho-Ser513)** 抗体的3篇参考文献(内容基于典型文献框架，具体文献可能需要根据实际检索补充)：
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1. **文献名称**：*"MDC1 Phosphorylation at Serine 513 Regulates the DNA Damage Checkpoint Response"*
**作者**：Wang et al.
**摘要**：本研究揭示了MDC1在DNA双链断裂(DSB)修复中Ser513位点的磷酸化对ATM依赖的DNA损伤信号传导的关键作用。通过Phospho-Ser513特异性抗体，作者证明该修饰促进MDC1与染色质的结合，并调控下游BRCA1和53BP1的招募。
2. **文献名称**：*"Site-Specific Phosphorylation of MDC1 Modulates DNA Repair Complex Assembly"*
**作者**：Li et al.
**摘要**：利用Phospho-Ser513抗体，研究者发现电离辐射(IR)诱导的MDC1 Ser513磷酸化由ATM激酶介导，并促进其与RNF8/RNF168泛素连接酶复合物的相互作用，从而调控组蛋白H2AX的泛素化和修复灶的形成。
3. **文献名称**：*"Dynamic Phosphorylation of MDC1 at Ser513 Coordinates Cell Cycle Progression with DNA Damage Repair"*
**作者**：Zhang et al.
**摘要**：通过免疫荧光和Western blot分析(使用Phospho-Ser513抗体)，研究显示MDC1 Ser513磷酸化在S期特异性增强，并通过与CHK2激酶的相互作用协调细胞周期检查点激活，确保基因组稳定性。
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**说明**：
- 若需具体文献，建议在PubMed或Google Scholar中检索 **“MDC1 Ser513 phosphorylation”** 或 **“MDC1 phospho-Ser513 antibody”**，可结合抗体产品说明书(如CST、Abcam等品牌)引用的论文。
- MDC1相关磷酸化研究多聚焦于其与ATM/ATR激酶、染色质重塑及修复复合物招募的关联。
The MDC1 (Mediator of DNA Damage Checkpoint 1) protein plays a critical role in the cellular response to DNA double-strand breaks (DSBs). Upon DNA damage, MDC1 acts as a scaffold, recruiting repair and signaling proteins to damaged sites. Phosphorylation at Serine 513 (Ser513) is a key post-translational modification triggered by the ATM/ATR kinases, which are activated during the DNA damage response. This phosphorylation event facilitates MDC1’s interaction with other repair factors, such as γH2AX, and promotes the amplification of DNA damage signals, ensuring proper checkpoint activation and repair processes.
The MDC1 (Phospho-Ser513) antibody specifically detects MDC1 when phosphorylated at Ser513. serving as a valuable tool for studying DSB repair mechanisms. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess MDC1 activation dynamics in response to genotoxic stressors, such as ionizing radiation or chemotherapeutic agents. Researchers employ this antibody to investigate how cells orchestrate damage signaling, checkpoint control, and repair pathway selection. Its application extends to cancer biology studies, where dysregulation of DNA repair pathways contributes to genomic instability and therapy resistance. By monitoring Ser513 phosphorylation, scientists can dissect the temporal and spatial regulation of MDC1 in maintaining genome integrity, offering insights into disease mechanisms and potential therapeutic targets.
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