| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AF1P; MLLT5; EPS15; Protein Eps15; |
| Entrez GeneID | 2060; |
| WB Predicted band size | 100kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EPS15 (Phospho-Tyr849)抗体的3篇参考文献摘要(基于公开文献的模拟示例)：
1. **文献名称**：*EPS15 phosphorylation regulates EGFR endocytosis in cancer cells*
**作者**：Smith A, et al.
**摘要**：研究揭示了EGFR信号通路中EPS15酪氨酸849位点(Y849)磷酸化对其内吞功能的调控机制，通过Phospho-Tyr849抗体证实该修饰在肿瘤细胞中异常激活，促进受体持续下游信号传导。
2. **文献名称**：*Tyrosine phosphorylation of EPS15 modulates clathrin-mediated endocytosis*
**作者**：Johnson R, et al.
**摘要**：利用EPS15 (Phospho-Tyr849)特异性抗体，作者发现Y849磷酸化通过增强EPS15与衔接蛋白AP2的结合能力，调控网格蛋白依赖的内吞过程，影响细胞膜受体再循环。
3. **文献名称**：*Phosphoproteomic analysis identifies EPS15-Y849 as a target of Src kinase in invasive breast cancer*
**作者**：Lee H, et al.
**摘要**：通过磷酸化蛋白质组学筛选，发现Src激酶诱导的EPS15-Y849磷酸化与乳腺癌转移相关，使用Phospho-Tyr849抗体验证其表达水平可作为预后标志物。
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**备注**：以上文献为示例性质，实际文献需通过PubMed或Google Scholar以关键词“EPS15 Tyr849 phosphorylation”或抗体货号查询。若该位点研究较少，可扩展至EPS15其他磷酸化位点或功能相关研究。
The EPS15 (Phospho-Tyr849) antibody is designed to detect EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15) when phosphorylated at tyrosine residue 849. a post-translational modification critical for its role in clathrin-mediated endocytosis and receptor tyrosine kinase signaling. EPS15 is an adaptor protein containing multiple protein-protein interaction domains, including EH (Eps15 homology) domains, which facilitate binding to ubiquitinated cargo and components of the endocytic machinery. Phosphorylation at Tyr849 occurs in response to growth factor stimulation (e.g., EGF) and is mediated by Src family kinases, enhancing EPS15's ability to regulate internalization of activated receptors like EGFR.
This antibody is widely used to study the activation status of EPS15 in cellular processes such as endocytosis, signal transduction, and receptor trafficking. It enables researchers to investigate the spatiotemporal dynamics of phosphorylated EPS15 in conditions like cancer, where dysregulated receptor endocytosis contributes to oncogenic signaling. Specificity for the phosphorylated Tyr849 epitope allows differentiation between active and inactive EPS15 forms, making it valuable in Western blotting, immunofluorescence, and immunoprecipitation applications. Its utility extends to exploring crosstalk between endocytic pathways and intracellular signaling networks, particularly in contexts involving tyrosine kinase inhibitors or Src-family kinase activity modulation.
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