| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | claudin 6; claudin-6; CLD6; Skullin 2; |
| Entrez GeneID | 9074; |
| WB Predicted band size | 23kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 219 (T-K-N-Y(p)-V) derived from Human Claudin 6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Claudin 6 (Phospho-Tyr219)抗体的参考文献示例(内容为模拟，仅供参考)：
1. **文献名称**：*"Phosphorylation of Claudin-6 at Tyrosine 219 Modulates Tight Junction Barrier Function in Breast Cancer"*
**作者**：Smith A, et al.
**摘要**：本研究通过开发特异性识别Claudin-6 Tyr219磷酸化位点的抗体，揭示了该位点的磷酸化通过激活Src激酶通路破坏紧密连接屏障功能，促进乳腺癌细胞侵袭和转移。
2. **文献名称**：*"Development and Validation of a Phospho-Specific Antibody for Claudin-6 (Tyr219) in Colorectal Cancer Models"*
**作者**：Zhang L, et al.
**摘要**：报道了一种针对Claudin-6 Tyr219磷酸化表位的单克隆抗体的开发过程，验证了其在结直肠癌组织中的特异性检测能力，并证明其磷酸化水平与患者预后不良相关。
3. **文献名称**：*"Tyrosine Phosphorylation of Claudin-6 Regulates Epithelial-Mesenchymal Transition in Gastric Epithelial Cells"*
**作者**：Tanaka K, et al.
**摘要**：利用Phospho-Tyr219抗体，阐明了Claudin-6在胃上皮细胞中的磷酸化通过调控E-cadherin表达诱导上皮-间质转化(EMT)，为胃癌治疗提供潜在靶点。
4. **文献名称**：*"Dynamic Phosphorylation of Claudin-6 at Tyr219 Drives Embryonic Stem Cell Differentiation"*
**作者**：Lee J, et al.
**摘要**：通过免疫沉淀和Western blot分析，发现Claudin-6 Tyr219的磷酸化在小鼠胚胎干细胞分化过程中动态变化，可能通过影响Wnt/β-catenin信号通路参与发育调控。
**注**：以上文献为示例，实际引用需根据真实发表的论文调整。建议通过PubMed或Google Scholar以关键词“Claudin 6 Phospho-Tyr219 antibody”检索最新研究。
The Claudin 6 (Phospho-Tyr219) antibody is a specialized tool used to detect the phosphorylation of Claudin 6 at tyrosine residue 219. a post-translational modification critical for regulating its function. Claudin 6. a member of the Claudin family, is a transmembrane protein integral to tight junction formation in epithelial and endothelial cells. These junctions control paracellular permeability, maintain cell polarity, and participate in cell signaling. Unlike some Claudins, Claudin 6 exhibits tissue-specific expression, often elevated during embryonic development and downregulated in adult tissues, though it is re-expressed in certain cancers, such as gastric, ovarian, and hepatocellular carcinomas.
Phosphorylation at Tyr219 modulates Claudin 6’s role in cell adhesion and signaling. Tyrosine phosphorylation can alter interactions with cytosolic scaffolding proteins, influence barrier function, or promote oncogenic signaling in cancer contexts. For example, phosphorylated Claudin 6 may enhance epithelial-mesenchymal transition (EMT) or metastasis by disrupting cell-cell adhesion. The Claudin 6 (Phospho-Tyr219) antibody enables researchers to study these dynamics in diseases, particularly cancer progression, drug resistance, or tissue integrity disorders. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation status in cell lines, tissues, or preclinical models. Validation of this antibody includes specificity checks via knockout controls or phosphopeptide competition assays, ensuring accurate detection of the active, phosphorylated form of Claudin 6.
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