Rabbit Polyclonal Claudin5(Phospho-Tyr217) Antibody

The Claudin 5 (Phospho-Tyr217) antibody is a specialized tool used to detect the phosphorylation status of tyrosine residue 217 in Claudin 5. a key component of tight junctions in endothelial cells. Claudin 5. a member of the Claudin family, is critical for maintaining blood-brain barrier (BBB) integrity by regulating paracellular permeability and cell-cell adhesion. Phosphorylation at Tyr217 is a post-translational modification that modulates Claudin 5’s interaction with cytoskeletal proteins or signaling molecules, influencing barrier dynamics. This modification may occur in response to physiological stimuli or pathological conditions, such as inflammation, ischemia, or neurodegenerative diseases, where BBB dysfunction is implicated. The antibody is widely employed in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study Claudin 5 regulation in vitro or in vivo. Researchers use it to explore mechanisms underlying endothelial barrier disruption or repair, particularly in neurological disorders, cancer metastasis, or vascular injury. Its specificity for the phosphorylated form allows precise tracking of Claudin 5 activity, aiding in the development of therapeutic strategies targeting tight junction plasticity. Proper validation, including knockout controls and phosphopeptide competition assays, is essential to ensure antibody specificity given the structural similarities among Claudin family members.