| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CTBP; C-terminal binding protein 1; EC 1.1.1; |
| Entrez GeneID | 1487; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 422(A-P-S(p)-P-G) derived from Human CtBP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CtBP1(Phospho-Ser422)抗体的参考文献示例(内容基于公开研究背景概括，非真实文献)：
1. **"Phosphorylation-dependent regulation of CtBP1 nuclear localization and transcriptional repression"**
- **作者**: Zhang Q, et al.
- **摘要**: 研究揭示了CtBP1在Ser422位点的磷酸化通过影响其与核转运蛋白的相互作用，调控其核质穿梭能力，并利用Phospho-Ser422抗体验证了DNA损伤条件下CtBP1的磷酸化状态变化。
2. **"CtBP1-Ser422 phosphorylation drives oncogenic gene expression in breast cancer"**
- **作者**: Lee J, et al.
- **摘要**: 在乳腺癌模型中，作者发现CtBP1的Ser422磷酸化通过解除对促癌基因(如MMP9)的抑制促进肿瘤侵袭，使用Phospho-Ser422抗体进行染色证实了磷酸化CtBP1在转移灶中的高表达。
3. **"Akt-mediated phosphorylation of CtBP1 modulates its interaction with HDACs and chromatin remodeling"**
- **作者**: Singh S, et al.
- **摘要**: 本文证明Akt激酶直接磷酸化CtBP1的Ser422位点，破坏其与组蛋白去乙酰化酶复合物(HDACs)的结合，进而影响染色质结构;Phospho-Ser422抗体用于检测Akt抑制剂处理后的磷酸化水平变化。
4. **"Metabolic stress-induced CtBP1 phosphorylation reprograms glucose metabolism in hepatocellular carcinoma"**
- **作者**: Wang Y, et al.
- **摘要**: 研究指出，低氧条件下CtBP1的Ser422磷酸化通过增强HIF-1α活性促进糖酵解，Phospho-Ser422抗体被用于肝癌组织芯片中磷酸化CtBP1与患者预后的相关性分析。
**注**：以上文献为示例性质，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar搜索关键词“CtBP1 Ser422 phosphorylation”或“CtBP1 pS422 antibody”获取具体文献。
CtBP1 (C-terminal binding protein 1) is a transcriptional co-repressor that regulates gene expression by interacting with DNA-binding proteins and chromatin-modifying enzymes. It plays roles in cellular processes such as apoptosis, differentiation, and metabolism, and is implicated in cancer progression. Phosphorylation at Ser422. located in its C-terminal region, modulates CtBP1's functional interactions. This post-translational modification reduces CtBP1's binding affinity for its partner proteins, including Hdm2 (human double minute 2), thereby influencing downstream pathways like p53-mediated tumor suppression.
The CtBP1 (Phospho-Ser422) antibody specifically detects CtBP1 when phosphorylated at serine 422. serving as a critical tool for studying its regulation and activity. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate phosphorylation-dependent changes in CtBP1 localization, protein-protein interactions, and transcriptional repression under various physiological or stress conditions. Its application has advanced understanding of how Ser422 phosphorylation impacts CtBP1's role in cancer metabolism, epithelial-mesenchymal transition (EMT), and responses to DNA damage. Validation of the antibody includes testing in cell lines with induced phosphorylation or site-directed mutagenesis to ensure specificity. Studies employing this reagent highlight the dynamic interplay between CtBP1 phosphorylation and its oncogenic or tumor-suppressive functions, making it valuable for both basic research and therapeutic target exploration.
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