| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CG1C; cyclin C; SRB11; |
| Entrez GeneID | 892; |
| WB Predicted band size | 37kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与Cyclin C (Phospho-Ser275)抗体相关的文献摘要信息：
1. **文献名称**：*Cyclin C is a haploinsufficient tumour suppressor*
**作者**：Wang et al.
**摘要**：研究发现Cyclin C磷酸化(Ser275)在DNA损伤后通过调控CDK8激酶活性参与转录暂停和细胞凋亡，其抗体用于检测DNA损伤修复中的磷酸化动态。
2. **文献名称**：*Oxidative stress induces nuclear-to-cytoplasmic translocation of Cyclin C*
**作者**：Cooper et al.
**摘要**：揭示了Ser275磷酸化是Cyclin C在氧化应激后从细胞核转位至线粒体的关键信号，使用该抗体验证了磷酸化修饰与细胞凋亡的关联性。
3. **文献名称**：*CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions*
**作者**：Haberle et al.
**摘要**：证明营养胁迫通过Ser275位点磷酸化调控Cyclin C-CDK8复合物的转录抑制功能，该抗体用于研究果蝇模型中磷酸化对代谢基因的调控作用。
Cyclin C, a member of the cyclin family, plays dual roles in regulating cell cycle progression and transcriptional modulation. It primarily associates with cyclin-dependent kinase 8 (CDK8) or CDK19 within the mediator complex, influencing RNA polymerase II-dependent gene expression. During stress or DNA damage, Cyclin C translocates to the mitochondria, promoting apoptosis by activating the mitochondrial fission pathway. The phosphorylation of Cyclin C at Ser275 is a critical post-translational modification that regulates its subcellular localization and functional switching between transcriptional co-regulation and apoptotic signaling. This phosphorylation event, mediated by stress-activated kinases, enhances Cyclin C's dissociation from the mediator complex and facilitates its mitochondrial targeting.
The Cyclin C (Phospho-Ser275) antibody is a specialized tool designed to detect endogenous Cyclin C protein when phosphorylated at serine 275. It is widely used in studies investigating cellular stress responses, DNA damage pathways, and cell cycle checkpoints. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation dynamics under various experimental conditions, including oxidative stress or chemotherapeutic treatments. Its specificity for the phosphorylated form makes it valuable for distinguishing active Cyclin C involved in apoptosis from its cell cycle-related functions. Validation typically includes knockout controls and peptide competition assays to ensure recognition of the precise phospho-epitope. This antibody contributes to understanding how Cyclin C phosphorylation coordinates cell fate decisions between survival and programmed death.
×
