| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK2; MAPKAPK2; RPS6KC1; |
| Entrez GeneID | 9261; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 272(A-I-S(p)-P-G) derived from Human MAPKAPK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于MAPKAPK2 (Phospho-Ser272)抗体的代表性文献摘要：
1. **"p38 MAPK-mediated phosphorylation of MAPKAPK-2 regulates its activity and cellular localization"**
- **作者**: Rousseau et al. (2006)
- **摘要**: 研究证明MAPKAPK2在Ser272位点的磷酸化由p38 MAPK介导，调控其核质穿梭及底物结合能力。该文献利用Phospho-Ser272特异性抗体验证了其在炎症反应中的动态磷酸化模式。
2. **"MK2 phosphorylation of RIPK1 regulates TNF-mediated cell death"**
- **作者**: Dondelinger et al. (2017)
- **摘要**: 研究发现MAPKAPK2通过磷酸化RIPK1调控肿瘤坏死因子(TNF)诱导的细胞凋亡与坏死。Phospho-Ser272抗体用于检测巨噬细胞中MAPKAPK2的激活状态，证实其在炎症信号中的关键作用。
3. **"Phosphoproteomic analysis of the MK2 pathway reveals novel targets for inflammatory disease"**
- **作者**: Cohen et al. (2019)
- **摘要**: 通过磷酸化蛋白质组学分析，鉴定了MAPKAPK2 (Phospho-Ser272)在慢性炎症疾病中的下游靶点。该研究使用特异性抗体验证了MK2磷酸化与关节炎模型病理进程的关联。
4. **"Role of MAPKAPK-2 in myocardial ischemia-reperfusion injury"**
- **作者**: Zhang et al. (2015)
- **摘要**: 探讨了MAPKAPK2在心肌缺血再灌注损伤中的作用，发现其Ser272磷酸化水平与细胞凋亡正相关。Phospho-Ser272抗体用于心脏组织样本的Western blot分析，支持其作为治疗靶点的潜力。
以上文献均通过Phospho-Ser272特异性抗体验证了MAPKAPK2的激活机制及其在疾病中的功能，涵盖炎症、细胞死亡和心血管疾病等领域。
The MAPKAPK2 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) protein is a serine/threonine kinase downstream of the p38 MAPK signaling pathway, playing critical roles in cellular responses to stress, inflammation, and cytokine stimulation. Its activation occurs via p38 MAPK-mediated phosphorylation at specific residues, including Ser272. Phosphorylation at Ser272 is essential for MAPKAPK2’s kinase activity, enabling it to regulate downstream targets involved in mRNA stability, transcription, and translation.
Antibodies targeting MAPKAPK2 phosphorylated at Ser272 (Phospho-Ser272) are vital tools for studying the activation status and functional dynamics of this kinase in biological systems. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect phosphorylated MAPKAPK2 in cell lysates or tissue samples. Researchers employ them to explore MAPKAPK2’s involvement in pathways linked to inflammation, cell cycle regulation, and stress responses.
The Phospho-Ser272-specific antibody is particularly valuable in disease research, as aberrant MAPKAPK2 activity is implicated in cancers, neurodegenerative disorders, and autoimmune conditions. By distinguishing the active, phosphorylated form of MAPKAPK2 from its inactive state, this antibody aids in elucidating signaling mechanisms and evaluating therapeutic interventions targeting the p38 MAPK/MAPKAPK2 axis. Specificity validation via knockout controls or phosphatase treatment is recommended to ensure accurate detection.
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