| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | pp100; GABJ; KIAA0571; |
| Entrez GeneID | 9846; |
| WB Predicted band size | 74kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Gab2 (Phospho-Tyr643)抗体相关的文献示例(内容为模拟概括，建议通过学术数据库核对原文)：
1. **《GRB2-associated binder 2 phosphorylation in cell migration regulation》**
- 作者：Suzuoki M, et al.
- 摘要：研究Gab2在Tyr643位点的磷酸化如何通过激活ERK/MAPK通路促进乳腺癌细胞迁移，抗体用于检测该位点的磷酸化水平与侵袭性表型的相关性。
2. **《Phosphorylation-dependent signaling of Gab2 in HER2-driven oncogenesis》**
- 作者：Qian X, et al.
- 摘要：探讨HER2过表达乳腺癌模型中Gab2 Tyr643磷酸化对PI3K-Akt和Ras-MAPK通路的激活作用，使用该抗体验证Gab2在肿瘤生长中的功能依赖性。
3. **《Site-specific phosphorylation of Gab2 by Src kinases regulates immune cell signaling》**
- 作者：Gu H, et al.
- 摘要：通过突变分析和抗体检测，证明Tyr643是Gab2在T细胞受体信号中的关键磷酸化位点，影响下游ZAP70和PLCγ的招募。
4. **《Gab2 tyrosine phosphorylation as a biomarker in leukemia progression》**
- 作者：Rodrigues GA, et al.
- 摘要：开发基于Phospho-Tyr643 Gab2抗体的检测方法，用于评估慢性髓系白血病患者中BCR-ABL融合蛋白的异常信号活性。
(注：以上为领域相关主题的模拟概括，实际文献需通过PubMed/Google Scholar以“Gab2 Tyr643 phosphorylation”为关键词检索。)
The Gab2 (Phospho-Tyr643) antibody is a specialized tool used to detect the phosphorylation of Gab2 (GRB2-associated binder 2) at tyrosine residue 643. Gab2 is a scaffolding adaptor protein involved in signal transduction pathways, particularly those activated by growth factor receptors, cytokine receptors, and immune receptors. Upon receptor activation, Gab2 becomes phosphorylated at specific tyrosine residues, including Tyr643. creating docking sites for downstream signaling molecules containing SH2 domains, such as phosphatidylinositol 3-kinase (PI3K) and SHP2 phosphatase. This phosphorylation event is critical for propagating signals through pathways like Ras/MAPK and PI3K/Akt, which regulate cell proliferation, differentiation, survival, and immune responses.
The Phospho-Tyr643 antibody specifically recognizes Gab2 when phosphorylated at this site, enabling researchers to study its activation status in various biological contexts. It is widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to investigate Gab2's role in physiological and pathological processes, including cancer, inflammation, and hematopoiesis. Dysregulation of Gab2 phosphorylation has been implicated in malignancies such as leukemia and breast cancer, making this antibody a valuable tool for both basic research and clinical studies. Its specificity ensures accurate detection of the active form of Gab2. aiding in the elucidation of signaling mechanisms and therapeutic targeting.
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