| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | pp100; KIAA0571 ; GRB2-associated binder 2; |
| Entrez GeneID | 9846; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of Serine 623(P-S-S(p)-P-S) derived from Human Gab2 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Gab2 (Phospho-Ser623)抗体的3篇示例参考文献(内容为虚构，仅供格式参考)：
1. **"Phosphorylation of Gab2 at Ser623 regulates ERK activation in breast cancer cells"**
- **作者**: Li et al.
- **摘要**: 研究揭示了Gab2蛋白Ser623位点的磷酸化在EGF信号通路中的关键作用，通过特异性抗体检测发现该修饰促进Gab2与SHP2结合，进而激活下游ERK通路，推动乳腺癌细胞增殖。
2. **"Role of Gab2 phosphorylation in leukemogenesis: Insights from Ser623 site-specific modulation"**
- **作者**: Martinez et al.
- **摘要**: 利用Phospho-Ser623抗体验证了慢性髓系白血病(CML)模型中Gab2的异常磷酸化，表明该位点受BCR-ABL激酶调控，并影响PI3K/AKT信号传导及白血病细胞存活。
3. **"Gab2 Ser623 phosphorylation mediates cellular migration in hepatocellular carcinoma"**
- **作者**: Wang et al.
- **摘要**: 通过免疫组化及Western blot分析，发现肝癌组织中Gab2 Ser623磷酸化水平升高，该修饰通过增强Gab2与MET受体相互作用，促进肿瘤细胞迁移和侵袭。
(注：以上文献为示例性内容，实际引用需查询真实数据库如PubMed。)
The Gab2 (Phospho-Ser623) antibody is a specialized tool used to detect the phosphorylation of Gab2 (GRB2-associated-binding protein 2) at serine residue 623. Gab2 is a scaffolding adaptor protein involved in signal transduction pathways downstream of receptor tyrosine kinases (RTKs), cytokine receptors, and immune receptors. It plays a critical role in regulating cellular processes such as proliferation, differentiation, and survival, primarily through interactions with signaling molecules like PI3K, SHP2. and Shc. Phosphorylation at Ser623 is a key post-translational modification that modulates Gab2 activity, influencing its ability to recruit downstream effectors and activate pathways like MAPK/ERK and PI3K/AKT. This modification is often associated with growth factor or cytokine stimulation and may regulate subcellular localization or protein-protein interactions.
The Gab2 (Phospho-Ser623) antibody is widely used in research to study Gab2 activation in contexts such as cancer, immune responses, and hematopoietic disorders. For example, hyperactivation of Gab2 has been linked to malignancies like breast cancer and leukemia, making this antibody valuable for investigating oncogenic signaling mechanisms. It is typically validated for applications including Western blotting, immunoprecipitation, and immunofluorescence. Researchers rely on its specificity to differentiate phosphorylated Gab2 from its unmodified form, enabling insights into dynamic signaling events. Proper experimental controls (e.g., phosphorylation inhibitors or site-directed mutagenesis) are recommended to confirm signal specificity. This antibody contributes to understanding disease mechanisms and potential therapeutic targets in pathways driven by Gab2 dysregulation.
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