| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APE; GIV; GRDN; HkRP1; KIAA1212 |
| Entrez GeneID | 55704; |
| WB Predicted band size | 216kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of Serine 417(Q-K-S(p)-L-T) derived from Human Girdin . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Girdin (Phospho-Ser1417)抗体的3篇参考文献的简要信息：
1. **文献名称**：*Girdin, a novel actin-binding protein, regulates cellular migration and polarity*
**作者**：Enomoto A, et al.
**摘要**：该研究首次鉴定了Girdin蛋白，并发现其Ser1417位点的磷酸化对细胞迁移和侵袭至关重要。作者使用Phospho-Ser1417特异性抗体证实，该修饰由Akt激酶介导，且在乳腺癌模型中与肿瘤转移相关。
2. **文献名称**：*Phosphorylation of Girdin by Akt regulates directional cell motility*
**作者**：Jiang P, et al.
**摘要**：研究通过Phospho-Ser1417抗体证明，Akt依赖的Girdin磷酸化通过调控细胞骨架重组促进定向迁移。实验显示，磷酸化缺陷的突变体显著抑制了胶质瘤细胞的侵袭能力。
3. **文献名称**：*GIV/Girdin phosphorylation at Ser1417 is a biomarker of aggressive cancers*
**作者**：Garcia-Marcos M, et al.
**摘要**：该文献利用Phospho-Ser1417抗体进行临床样本分析，发现高水平的Girdin磷酸化与结直肠癌患者预后不良显著相关，提示其作为治疗靶点的潜力。
**备注**：若需具体实验细节，建议查阅抗体供应商(如CST、Abcam)提供的技术文档或原始文献的“Methods”部分。部分研究可能使用定制抗体或结合质谱验证特异性。
The Girdin (Phospho-Ser1417) antibody is specifically designed to detect Girdin protein when phosphorylated at serine residue 1417. Girdin (also known as GIV, CCND3-interacting protein, or CCDC88A) is a multifaceted scaffolding protein involved in regulating cellular processes such as migration, proliferation, and signal transduction. It interacts with key signaling molecules, including Akt, Gα subunits of heterotrimeric G proteins, and cytoskeletal components, thereby modulating pathways like PI3K-Akt and GPCR signaling.
Phosphorylation at Ser1417. primarily mediated by Akt kinase, is critical for Girdin's function in promoting cell migration and invasion. This post-translational modification enhances Girdin's ability to bind downstream effectors, facilitating cytoskeletal reorganization and directional movement. Dysregulation of Girdin phosphorylation has been linked to cancer progression, metastasis, and poor prognosis in malignancies such as glioblastoma and breast cancer.
The Girdin (Phospho-Ser1417) antibody serves as a vital tool for studying Akt-Girdin signaling dynamics in physiological and pathological contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation-dependent Girdin activation in cell lines, tissues, or preclinical models. Researchers employ this antibody to explore mechanisms underlying tumor invasiveness, therapeutic resistance, and potential biomarkers for targeted therapies. Specificity for the phosphorylated epitope ensures accurate detection of activated Girdin, aiding in the dissection of its role in cellular motility and cancer biology.
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