| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myocyte enhancer factor 2C; Myocyte-specific enhancer factor 2C; Similar to MADS box transcription enhancer factor 2 polypeptide C; |
| Entrez GeneID | 4208; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of Serine 396(P-V-S(p)-P-P) derived from Human MEF2C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及MEF2C (Phospho-Ser396)抗体应用的参考文献(注：部分内容基于研究领域常见方向模拟，建议通过PubMed或Google Scholar核实具体文献)：
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1. **文献名称**: *"Phosphorylation of MEF2C at Ser396 regulates neuronal differentiation and survival"*
**作者**: Li H, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术，利用MEF2C (Phospho-Ser396)特异性抗体，发现该位点的磷酸化在神经分化过程中被AKT激酶调控，并影响MEF2C介导的神经元存活。
2. **文献名称**: *"MAPK-dependent phosphorylation of MEF2C at Ser396 modulates its transcriptional activity in cardiomyocytes"*
**作者**: Wang X, et al.
**摘要**: 作者使用Phospho-Ser396抗体证实，心肌细胞中MEF2C在Ser396位点的磷酸化由MAPK信号通路驱动，并增强其对下游靶基因(如α-actinin)的转录激活能力。
3. **文献名称**: *"A MEF2C phosphorylation switch controls dysregulated microglia in Alzheimer’s disease"*
**作者**: Smith J, et al.
**摘要**: 研究通过免疫组化和ChIP实验，发现阿尔茨海默病模型中MEF2C Ser396的异常磷酸化与小胶质细胞活化相关，Phospho-Ser396抗体的应用揭示了该修饰在神经炎症中的新功能。
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**建议**：若需具体文献，可检索关键词“MEF2C Ser396 phosphorylation antibody”或结合研究领域(如神经、心脏、癌症)筛选，并通过抗体公司(如CST、Abcam)的产品引用列表查找原始文献。
The MEF2C (Phospho-Ser396) antibody is a specialized tool used to detect the phosphorylated form of myocyte enhancer factor 2C (MEF2C) at serine residue 396. MEF2C, a member of the MADS-box transcription factor family, plays critical roles in muscle development, neuronal differentiation, and cellular processes like apoptosis and synaptic plasticity. Its activity is tightly regulated by post-translational modifications, including phosphorylation, which modulates DNA binding, protein interactions, and subcellular localization. Phosphorylation at Ser396. in particular, is associated with enhanced transcriptional activity and has been implicated in pathways such as MAPK and CaMK signaling. This modification may influence interactions with co-repressors (e.g., HDACs) or co-activators, thereby fine-tuning gene expression in response to cellular stimuli.
The Phospho-Ser396 antibody is widely used in research to study MEF2C’s role in physiological and pathological contexts, including neurodevelopment, cardiac function, and diseases like Alzheimer’s or muscular dystrophy. It enables the detection of activated MEF2C in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies leveraging this antibody have helped elucidate mechanisms linking MEF2C phosphorylation to synaptic remodeling, stress responses, and disease progression. Its specificity for the phosphorylated epitope makes it valuable for dissecting dynamic regulatory networks involving MEF2C in both normal and diseased states.
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