| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NDF1; NEUROD; NeuroD1; beta2; |
| Entrez GeneID | 4760; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 274(P-L-S(p)-P-P) derived from Human Neuro D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Neuro D (Phospho-Ser274)抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*Phosphorylation of NeuroD at Serine 274 Regulates Neuronal Differentiation*
**作者**：Smith et al.
**摘要**：研究揭示NeuroD在Ser274位点的磷酸化通过MAPK信号通路调控神经前体细胞的分化。抗体用于Western blot和免疫荧光，证明该位点磷酸化对神经突生长至关重要。
2. **文献名称**：*Post-translational Modification of NeuroD1 Impacts Hippocampal Neurogenesis*
**作者**：Chen & Lee
**摘要**：探讨NeuroD1翻译后修饰对海马神经发生的影响。通过Phospho-Ser274抗体发现，该位点磷酸化受AKT通路调控，并与记忆形成相关。
3. **文献名称**：*Role of NeuroD Phosphorylation in Pancreatic β-cell Function*
**作者**：Johnson et al.
**摘要**：研究NeuroD在胰岛β细胞中的功能，使用Phospho-Ser274抗体证实其磷酸化水平与胰岛素分泌相关，提示其在糖尿病病理中的潜在作用。
注：上述文献为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议用关键词“NeuroD1 Phospho-Ser274”或“NeuroD Ser274 phosphorylation”进一步查找。
Neuro D (Phospho-Ser274) antibody is a specialized tool used to detect the phosphorylated form of NeuroD, a transcription factor critical in neuronal development and differentiation. NeuroD, encoded by the *NEUROD1* gene, belongs to the basic helix-loop-helix (bHLH) family and plays pivotal roles in neurogenesis, pancreatic beta-cell maturation, and synaptic plasticity. Phosphorylation at serine 274 (Ser274) modulates NeuroD's activity, influencing its ability to bind DNA or interact with co-regulators, thereby regulating target gene expression. This post-translational modification is often associated with cellular signaling pathways, such as MAPK or AKT, which are activated during developmental processes or stress responses.
The Neuro D (Phospho-Ser274) antibody is designed to recognize NeuroD specifically when phosphorylated at Ser274. enabling researchers to study its activation status in various contexts. It is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess NeuroD's role in neuronal differentiation, insulin production in pancreatic cells, or its dysregulation in diseases like diabetes, neurodegenerative disorders, or cancer. Validation typically includes testing on cell lines or tissue samples with known phosphorylation status, and specificity is confirmed via knockout controls or peptide competition assays. This antibody serves as a key reagent for exploring NeuroD's dynamic regulation in health and disease.
×
