| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | polypeptide 5; KS6A5; MSPK1; RPS6KA5; RLPK |
| Entrez GeneID | 9252; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 212 (A-Y-S(p)-F-C) derived from Human MSK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MSK1 (Phospho-Ser212)抗体的3篇参考文献及其简要摘要：
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1. **Title**: *"Regulation of MSK1 activity by phosphorylation and its role in chromatin remodeling"*
**Authors**: Soloaga A. et al.
**Summary**: 该研究通过体外激酶实验和免疫印迹分析，利用Phospho-Ser212抗体证明MSK1在丝裂原刺激下通过ERK/p38通路被磷酸化，并揭示其磷酸化状态对组蛋白H3修饰和基因转录调控的关键作用。
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2. **Title**: *"MSK1-mediated phosphorylation of CREB in neuronal plasticity and memory formation"*
**Authors**: Arthur J.S.C. et al.
**Summary**: 文章使用Phospho-Ser212特异性抗体检测神经元中MSK1的激活，发现其通过磷酸化CREB蛋白参与突触可塑性和长期记忆形成，且该过程依赖Ser212位点的磷酸化。
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3. **Title**: *"Distinct roles of MSK1 and MSK2 in MAPK-induced chromatin remodeling"*
**Authors**: Deak M. et al.
**Summary**: 研究通过免疫沉淀和免疫荧光技术，结合Phospho-Ser212抗体，比较MSK1与MSK2在MAPK信号中的差异，发现MSK1的Ser212磷酸化是其核定位及调控炎症基因表达的必要条件。
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**备注**：若需具体文献来源(期刊、年份等)，建议在PubMed或抗体供应商(如CST #9591)的产品引用列表中进一步检索。
The MSK1 (Phospho-Ser212) antibody is a specialized tool used to detect the activated form of Mitogen and Stress-Activated Protein Kinase 1 (MSK1) when phosphorylated at serine 212. MSK1. a Ser/Thr kinase, plays a critical role in mediating cellular responses to extracellular stimuli such as growth factors, stress, or cytokines. It is primarily activated downstream of the MAPK/ERK or p38 MAPK signaling pathways. Phosphorylation at Ser212. located within the kinase domain’s activation loop, is essential for MSK1’s catalytic activity, enabling it to phosphorylate downstream targets like CREB (cAMP-response element-binding protein) and histone H3. thereby regulating gene expression linked to proliferation, survival, and inflammatory responses.
This antibody is widely used in techniques such as Western blotting, immunofluorescence, or immunoprecipitation to study MSK1 activation dynamics in response to stimuli like UV radiation, osmotic stress, or growth factors. Researchers employ it to investigate MSK1’s role in chromatin remodeling, transcriptional regulation, and disease contexts such as cancer, neurodegeneration, or immune disorders. Specificity for the phosphorylated Ser212 residue ensures accurate detection of the active kinase, aiding in understanding signaling crosstalk and therapeutic targeting of MSK1-associated pathways. Proper controls, including knockout cells or phosphatase treatment, are recommended to confirm signal specificity.
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