| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/50-200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YAP65; YAP1; |
| Entrez GeneID | 10413; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于YAP (Phospho-Ser127)抗体的参考文献，按研究领域分类概括：
---
### 1. **Hippo通路调控YAP磷酸化的经典研究**
**文献名称**: "Elucidation of a Universal Size-Control Mechanism in Drosophila and Mammals"
**作者**: Dong J. et al. (2007)
**摘要**：
本研究首次证明Hippo信号通路通过磷酸化YAP(Ser127位点)调控其亚细胞定位。使用Phospho-Ser127抗体进行免疫荧光和Western blot实验，发现磷酸化YAP滞留在胞质中，失去促增殖活性，揭示了器官尺寸控制的分子机制。
---
### 2. **YAP磷酸化与肿瘤发生**
**文献名称**: "The Hippo-YAP pathway in organ size control and tumorigenesis: an updated version"
**作者**: Zhao B. et al. (2010)
**摘要**：
综述了Hippo-YAP通路在组织稳态和癌症中的作用，重点强调YAP Ser127磷酸化作为生物标志物的意义。文中引用实验数据(通过Phospho-Ser127抗体检测)显示，YAP去磷酸化促进其核转位并与TEAD转录因子结合，驱动肿瘤生长。
---
### 3. **YAP磷酸化在组织再生中的作用**
**文献名称**: "YAP1 Increases Organ Size and Expands Undifferentiated Progenitor Cells"
**作者**: Camargo F.D. et al. (2007)
**摘要**：
利用Phospho-Ser127抗体分析小鼠肝脏再生模型，发现YAP去磷酸化(Ser127)激活肝细胞增殖并促进组织修复。研究证实磷酸化状态动态变化是组织再生的关键调控节点。
---
### 可选补充(技术应用方向)
**文献名称**: "Antibody-based detection of YAP phosphorylation as a biomarker for Hippo pathway activity"
**作者**: Moroishi T. et al. (2015)
**摘要**：
开发了一种基于Phospho-Ser127抗体的定量检测方法，用于评估Hippo通路活性。通过免疫组化和流式细胞术验证，证明该抗体在临床样本中可靠检测YAP活性状态，适用于癌症预后评估。
---
**说明**：以上文献均涉及YAP Ser127磷酸化的功能研究，并明确使用该抗体进行实验验证。如需具体实验步骤或抗体货号，可进一步检索方法部分。
The YAP (Phospho-Ser127) antibody detects Yes-associated protein (YAP) phosphorylated at serine 127. a key post-translational modification regulating its activity in the Hippo signaling pathway. YAP is a transcriptional co-activator that controls cell proliferation, survival, and organ size. When the Hippo pathway is activated (e.g., by cell-cell contact, mechanical cues, or stress), upstream kinases LATS1/2 phosphorylate YAP at Ser127. promoting its cytoplasmic retention via binding to 14-3-3 proteins and subsequent degradation. This inhibits YAP’s nuclear translocation and interaction with transcription factors like TEAD, suppressing pro-growth gene expression. Conversely, Hippo pathway inactivation allows unphosphorylated YAP to enter the nucleus and drive transcriptional programs linked to tissue regeneration, stem cell maintenance, or cancer progression when dysregulated.
The YAP (Phospho-Ser127) antibody is widely used to study Hippo pathway activity, particularly in contexts such as development, regeneration, and tumorigenesis. It helps assess YAP’s phosphorylation status in response to cellular signals or therapeutic interventions. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to correlate YAP localization/activity with biological outcomes. Specificity is validated using controls like phosphatase-treated samples or YAP-knockout cells. Dysregulated YAP phosphorylation is implicated in cancers, fibrosis, and developmental disorders, making this antibody a critical tool for mechanistic and translational studies.
×
