Rabbit Polyclonal NMDAR1(Phospho-Ser890) Antibody

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     Western blot analysis of NMDAR1 (pS890) expression in A549 (A), U87MG (B) whole cell lysates.    

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     Immunofluorescent analysis of NMDAR1 (pS890) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.    

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     Immunohistochemical analysis of NMDAR1 (pS890) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.    

The NMDAR1 (Phospho-Ser890) antibody is a specialized tool used to detect the phosphorylation state of the NMDA (N-methyl-D-aspartate) receptor subunit GluN1 (NR1) at serine residue 890. NMDA receptors are ionotropic glutamate receptors critical for synaptic plasticity, learning, and memory. The NR1 subunit is essential for receptor assembly and function, with post-translational modifications like phosphorylation regulating receptor trafficking, localization, and activity. Phosphorylation at Ser890. located within the intracellular C-terminal domain of NR1. is mediated by protein kinase C (PKC) and influences receptor internalization. This site-specific modification disrupts interactions with scaffolding proteins, promoting clathrin-dependent endocytosis of NMDA receptors, thereby modulating synaptic strength.

The Phospho-Ser890 antibody is widely used in neuroscience research to study activity-dependent changes in receptor distribution, particularly in contexts like long-term depression (LTD), excitotoxicity, or neurological disorders. It enables detection of dynamic phosphorylation events in brain tissue or cultured neurons through techniques like Western blotting, immunohistochemistry, or immunofluorescence. Studies using this antibody have highlighted its role in linking PKC signaling to synaptic NMDA receptor availability, offering insights into mechanisms underlying neuropsychiatric diseases, epilepsy, and ischemic injury. Proper validation with phosphorylation-deficient mutants or PKC inhibitors is recommended to ensure specificity.