| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein kinase; CAMK group; CAMKL family; LKB subfamily; |
| Entrez GeneID | 6794; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine428 (R-L-S(p)-A-C) derived from Human LKB1 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及LKB1 (Phospho-Ser428)抗体应用的文献摘要示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**: "LKB1 phosphorylation at Ser428 modulates AMPK signaling in energy stress"
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot使用Phospho-Ser428特异性抗体，发现能量应激条件下LKB1在Ser428位点的磷酸化水平显著升高，激活下游AMPK通路，揭示了该磷酸化事件在代谢调控中的关键作用。
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2. **文献名称**: "Role of LKB1-Ser428 phosphorylation in cell polarity regulation"
**作者**: Chen L, et al.
**摘要**: 作者利用Phospho-Ser428抗体进行免疫荧光实验，证明LKB1的Ser428磷酸化是其定位于细胞前沿并调控上皮细胞极性的必要条件，突变该位点会破坏极性相关信号通路。
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3. **文献名称**: "Validation of a phospho-specific antibody for LKB1 Ser428 in cancer models"
**作者**: Wang X, et al.
**摘要**: 本研究系统验证了LKB1 (Phospho-Ser428)抗体的特异性，发现其在肺癌组织中可区分磷酸化与非磷酸化LKB1.并证实该位点磷酸化缺失与肿瘤侵袭性相关。
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**注意**：以上文献为示例性质，实际文献需通过PubMed或Google Scholar以关键词"LKB1 Ser428 phosphorylation antibody"检索。推荐查阅以下真实期刊中的相关研究：
- *Cell Metabolism*(代谢与信号转导)
- *Journal of Biological Chemistry*(分子机制研究)
- *Oncogene*(癌症相关磷酸化研究)
The LKB1 (Phospho-Ser428) antibody is a specialized tool used to detect the phosphorylated form of the LKB1 protein at serine residue 428. LKB1 (Liver kinase B1), also known as STK11. is a serine/threonine kinase that functions as a tumor suppressor and regulates cellular processes such as metabolism, polarity, and proliferation. Phosphorylation at Ser428 is a critical post-translational modification linked to LKB1’s activation, subcellular localization, and interaction with downstream targets like AMP-activated protein kinase (AMPK), a key regulator of energy homeostasis.
This antibody is widely employed in research to study LKB1 signaling pathways, particularly in contexts of cancer, metabolic disorders (e.g., diabetes), and Peutz-Jeghers syndrome, a condition caused by LKB1 mutations. By specifically recognizing the phosphorylated Ser428 epitope, it enables the assessment of LKB1 activation status in experimental models using techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers often use it to investigate how LKB1 phosphorylation impacts cellular responses to stress, energy deprivation, or therapeutic agents.
Validation of the antibody’s specificity is essential, as non-phosphorylated LKB1 or cross-reactivity with other phospho-proteins can lead to false results. Proper sample preparation, including phosphatase inhibitors, is required to preserve phosphorylation signals during experiments. Its applications extend to both basic mechanistic studies and translational research aiming to target LKB1-related pathways in disease.
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