| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EPO R; EPO-R; epor; |
| Entrez GeneID | 2057; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 368 (D-T-Y(p)-L-V) derived from Human Epo-R. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Epo-R (Phospho-Tyr368)抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **"JAK2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin"**
*Authors: Witthuhn BA, Quelle FW, Silvennoinen O, Yi T, Tang B, Miura O, Ihle JN*
**摘要**: 本研究揭示了Epo受体(Epo-R)与JAK2激酶的相互作用，证明Epo刺激后Epo-R的酪氨酸残基(包括Tyr368)发生磷酸化，触发JAK2激活，进而启动下游信号通路(如STAT5)。该研究为Epo-R磷酸化在信号传导中的核心作用提供了证据。
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2. **"Tyrosine phosphorylation and activation of STAT5. STAT3. and Janus kinases by Epo receptors"**
*Authors: Klingmüller U, Lorenz U, Cantley LC, Neel BG, Lodish HF*
**摘要**: 通过突变Epo-R的特定酪氨酸位点(如Tyr368)，发现其磷酸化对STAT5的招募和激活至关重要。研究利用磷酸化特异性抗体验证Tyr368位点的修饰，表明该位点是JAK2/STAT5信号轴的关键调控点。
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3. **"Erythropoietin receptor phosphorylation by Lyn kinase regulates hematopoietic progenitor cell cycles and survival"**
*Authors: Sasaki A, Yasukawa H, Shouda T, Kitamura T, Dikic I, Yoshimura A*
**摘要**: 通过抗Epo-R (Phospho-Tyr368)抗体的Western blot分析，证实Epo刺激后Tyr368位点的磷酸化由Lyn激酶介导，并调控造血干细胞的增殖与存活。研究揭示了该磷酸化事件在红系分化中的新机制。
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**备注**：上述文献为示例性质，实际引用时需核实数据库(如PubMed)中的准确性。若需具体文章，建议通过关键词“Epo-R Tyr368 phosphorylation antibody”或相关信号通路进一步检索。
The Epo-R (Phospho-Tyr368) antibody is designed to detect the phosphorylated form of the erythropoietin receptor (Epo-R) at tyrosine residue 368. a critical post-translational modification involved in Epo-mediated signaling. Epo-R, a member of the cytokine receptor superfamily, is activated upon binding of erythropoietin (Epo), a hormone essential for red blood cell production. Ligand binding induces receptor dimerization and activation of associated Janus kinase 2 (JAK2), which phosphorylates specific tyrosine residues on Epo-R, including Tyr368. This phosphorylation creates docking sites for downstream signaling adaptors, such as STAT5. which propagate signals regulating cell survival, proliferation, and differentiation in erythroid progenitor cells.
Phosphorylation at Tyr368 is a hallmark of Epo-R activation and is tightly regulated. Dysregulation of this pathway is linked to disorders like polycythemia vera and certain cancers, where aberrant Epo-R signaling drives uncontrolled cell growth. The Epo-R (Phospho-Tyr368) antibody serves as a vital tool for studying receptor activation dynamics, signal transduction mechanisms, and pathological conditions. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation status in experimental models, aiding research into therapeutic strategies targeting Epo-R-JAK-STAT signaling. Specificity for the phosphorylated Tyr368 epitope ensures precise detection of the active receptor form, distinguishing it from the inactive state.
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