关键信息
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基因名
PPARd
- 应用
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别名
PPARd；NR1C2；PPARB；Peroxisome proliferator-activated receptor delta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q03181
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表达区间
165-441aa
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氨基酸序列
GSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGDRPGLMNVPRVEAIQDTILRALEFHLQANHPDAQYLFPKLLQKMADLRQLVTEHAQMMQRIKKTETETSLHPLLQEIYKDMY
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分子量
49.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Peroxisome proliferator-activated receptors (PPARs) are a family of nuclear receptor proteins that function as transcription factors regulating various biological processes, including lipid metabolism, glucose homeostasis, and inflammation. Among them, PPAR delta (PPARd) has garnered significant attention due to its role in fatty acid oxidation and energy homeostasis. Research has shown that PPARd activation can enhance metabolic flexibility, making it a potential therapeutic target for metabolic disorders such as obesity, diabetes, and cardiovascular diseases. The recombinant protein of PPARd is critical for understanding its structure-function relationships and for elucidating the molecular mechanisms through which it exerts its effects on gene expression. By producing and studying the PPARd recombinant protein, scientists aim to unravel its interactions with specific co-regulators and ligands, paving the way for the development of targeted drugs. Recent advancements in recombinant DNA technology have enabled the efficient production of PPARd in various expression systems, facilitating in vitro and in vivo studies. Such research is essential not only for basic science but also for translating findings into clinical applications, potentially offering new strategies for managing metabolic syndromes.
