| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAY1, PKCD, nPKC-delta |
| Entrez GeneID | 5580; |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 313 (G-I-Y(p)-Q-G) derived from Human PKC delta. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PKCδ (Phospho-Tyr313)抗体的参考文献及其摘要概括：
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1. **文献名称**: *Tyrosine phosphorylation of protein kinase Cδ is essential for its apoptotic effect in response to etoposide*
**作者**: Li W. et al.
**摘要**: 研究揭示了DNA损伤药物依托泊苷诱导PKCδ在Tyr313位点的磷酸化，并通过该抗体验证其磷酸化状态。磷酸化的PKCδ促进其核转位，激活caspase-3.最终导致细胞凋亡。
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2. **文献名称**: *Role of PKCδ tyrosine phosphorylation in oxidative stress-induced cell death*
**作者**: Steinberg S.F. et al.
**摘要**: 本文利用Phospho-Tyr313抗体证明，氧化应激(如H2O2处理)通过Src家族激酶介导PKCδ的Tyr313磷酸化，触发线粒体依赖性凋亡通路，在心肌细胞损伤中起关键作用。
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3. **文献名称**: *Phosphorylation of PKCδ Tyr313 correlates with podocyte injury in diabetic nephropathy*
**作者**: Liu Y. et al.
**摘要**: 通过该抗体检测发现，高糖环境下肾足细胞中PKCδ Tyr313磷酸化水平升高，导致足细胞骨架蛋白分解和凋亡，提示其在糖尿病肾病中的病理机制。
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4. **文献名称**: *Tyrosine 313 phosphorylation enhances PKCδ proteolytic activation during DNA damage response*
**作者**: Blass M. et al.
**摘要**: 研究利用Phospho-Tyr313抗体证实，电离辐射诱导PKCδ在Tyr313位点磷酸化，促进其被caspase-3切割生成促凋亡片段，为癌症治疗中DNA损伤响应机制提供了新见解。
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以上文献均通过Phospho-Tyr313抗体验证PKCδ在特定病理或生理条件下的功能机制，涉及凋亡、氧化应激及疾病模型。
The PKC delta (Phospho-Tyr313) antibody is designed to detect protein kinase C delta (PKCδ) when phosphorylated at tyrosine residue 313 (Tyr313). PKCδ, a member of the novel PKC subfamily, is a serine/threonine kinase involved in diverse cellular processes, including apoptosis, differentiation, and proliferation. Its activity is tightly regulated by post-translational modifications, such as phosphorylation. Phosphorylation at Tyr313. mediated by Src family kinases or other tyrosine kinases, plays a critical role in PKCδ activation and subcellular localization. This modification is implicated in stress-induced signaling pathways, DNA damage responses, and receptor-mediated signaling.
The antibody is widely used in research to study PKCδ activation dynamics in contexts like oxidative stress, DNA-damaging agents (e.g., UV radiation, chemotherapeutics), and growth factor signaling. Applications include Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry. Specificity is validated using phosphorylation-deficient mutants or phosphatase-treated samples. Dysregulation of PKCδ Tyr313 phosphorylation has been linked to cancer progression, neurodegenerative diseases, and immune disorders, making this antibody a valuable tool for investigating molecular mechanisms in pathophysiology. Proper controls are essential to distinguish signal specificity from cross-reactivity with other phospho-tyrosine epitopes.
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