Rabbit Polyclonal BCL-2(Phospho-Thr69) Antibody

The BCL-2 (Phospho-Thr69) antibody is a specialized tool used to detect B-cell lymphoma 2 (BCL-2) protein phosphorylated at threonine 69 (Thr69), a post-translational modification linked to its functional regulation. BCL-2 is a key anti-apoptotic protein that prevents mitochondrial outer membrane permeabilization, thereby inhibiting programmed cell death. Its activity is tightly controlled by phosphorylation, which can modulate interactions with pro-apoptotic BCL-2 family members (e.g., BAX, BAK) or alter protein stability. Phosphorylation at Thr69 has been implicated in stress-induced apoptotic signaling, particularly in response to DNA damage or chemotherapeutic agents. This site-specific phosphorylation may influence BCL-2’s ability to sequester pro-death proteins or regulate autophagic processes. The antibody is widely used in cancer research, as BCL-2 overexpression is associated with tumor survival, chemoresistance, and poor prognosis. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, or flow cytometry to study BCL-2 phosphorylation dynamics in cellular models or patient samples. Validation often includes testing in apoptosis-inducing conditions (e.g., UV irradiation, staurosporine treatment) to confirm phosphorylation-dependent detection. Understanding Thr69 phosphorylation provides insights into therapeutic strategies targeting BCL-2 dysregulation in cancers or neurodegenerative diseases.