Rabbit Polyclonal AurB/C(Phospho-Thr236/202) Antibody

The Aurora kinase family, comprising Aurora A, B, and C, plays a critical role in regulating mitotic progression, particularly chromosome alignment, spindle assembly, and cytokinesis. Aurora B and C, closely related paralogs, share high sequence homology and functional overlap, though Aurora C is primarily active during meiosis and in certain somatic tissues. The AurB/C (Phospho-Thr236/202) antibody specifically detects the phosphorylated forms of Aurora B (at Thr236) and Aurora C (at Thr202), residues critical for their catalytic activation. Phosphorylation at these sites is essential for kinase activity, enabling recruitment to centromeres, interaction with coactivators like INCENP, and regulation of the spindle assembly checkpoint. Dysregulation of Aurora B/C is linked to mitotic errors, genomic instability, and cancer progression, making them targets for anticancer therapies. This antibody is widely used in research to study mitotic dynamics, checkpoint control, and drug response in cancer models. It is validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to assess Aurora B/C activation status in cell lines or tissues. Its specificity for phosphorylated forms allows researchers to distinguish active kinases from inactive pools, providing insights into cell cycle regulation and therapeutic targeting.