| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H1 antibody; HIST1H1B antibody; Histone 1 antibody; Histone H1a antibody |
| Entrez GeneID | 3006; |
| WB Predicted band size | 17-25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 1(M-S(p)-E-T) derived from Human Histone H1. |
| Formulation | Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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以下是3篇关于Histone H1(Phospho-Ser1)抗体的模拟参考文献(非真实文献，供参考格式)：
1. **文献名称**：*Phosphorylation of Histone H1 at Serine 1 Modulates Chromatin Condensation during Mitosis*
**作者**：Zhang L, et al.
**摘要**：研究揭示了组蛋白H1在Ser1位点的磷酸化对有丝分裂期间染色质凝聚的关键作用，通过特异性Rabbit抗体检测发现其磷酸化水平在G2/M期显著上调。
2. **文献名称**：*A Novel Role of Histone H1 Phosphorylation in DNA Damage Response*
**作者**：Kimura T, et al.
**摘要**：利用Phospho-Ser1 H1抗体，研究证明该修饰参与DNA损伤修复过程，磷酸化H1通过促进染色质松弛增强修复蛋白的招募。
3. **文献名称**：*Development and Validation of a Phospho-Specific Antibody for Histone H1 Serine 1*
**作者**：Roberts JM, et al.
**摘要**：文献描述了Phospho-Ser1 H1兔多克隆抗体的开发流程，验证其在免疫印迹和免疫荧光中的特异性，并应用于癌症细胞周期研究。
注：以上文献为模拟内容，实际引用需查询PubMed或SciFinder等数据库获取真实文献。
Histone H1 (Phospho-Ser1) Rabbit antibody is a specialized tool used to study the phosphorylation state of histone H1 at serine residue 1 (Ser1), a post-translational modification implicated in chromatin dynamics and gene regulation. Histone H1. a linker histone, binds to nucleosomes and facilitates higher-order chromatin compaction. Its phosphorylation, particularly at Ser1. is associated with cell cycle progression, especially during mitosis, where chromatin condensation and decondensation are tightly regulated. This modification is thought to weaken histone-DNA interactions, promoting chromatin relaxation during transcription or replication, and re-establishing compact structures post-mitosis.
The antibody is generated by immunizing rabbits with a synthetic phosphopeptide corresponding to the Ser1 phosphorylation site of histone H1. ensuring specificity for the phosphorylated epitope. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect and quantify Ser1-phosphorylated histone H1 in various biological samples, including cell lines and tissues. Researchers employ this antibody to investigate epigenetic mechanisms, cell cycle checkpoints, and DNA damage responses, as dysregulated histone H1 phosphorylation has been linked to cancers, developmental disorders, and chromatin-related diseases. Proper validation, including knockout controls and phosphatase treatment, is critical to confirm signal specificity, as cross-reactivity with non-phosphorylated histone H1 or other phospho-epitopes may occur.
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