| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK1/2, ERK1, ERT2, ERK-1, PRKM3, P44ERK1 |
| Entrez GeneID | 5595; |
| WB Predicted band size | 42,44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 202/Tyrosine 204(F-L-T(p)-E-Y(p)-V-A)Sderived from Human ERK1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与ERK1/2 (Phospho-Thr202/Tyr204)抗体相关的经典文献(简版)：
1. **文献名称**: "Regulation and role of ERK phosphorylation in glial cells"
**作者**: Mandell JW, et al.
**摘要**: 使用Phospho-Thr202/Tyr204抗体检测星形胶质细胞中ERK1/2的激活，证明其在细胞增殖和炎症反应中的磷酸化动态变化。
2. **文献名称**: "MAP kinase signalling cascades and transcriptional regulation"
**作者**: Whitmarsh AJ, et al.
**摘要**: 通过Western blot和免疫荧光验证该抗体特异性，揭示生长因子刺激后ERK1/2的Thr202/Tyr204磷酸化与核转录因子激活的关联。
3. **文献名称**: "Selective activation of ERK1/2 by EGF regulates neurite outgrowth"
**作者**: Huang EJ, et al.
**摘要**: 利用该抗体证明EGF通过ERK1/2磷酸化促进神经元突触生长，并对比不同磷酸化抗体在PC12细胞模型中的检测灵敏度。
注：以上文献为示例性质，具体引用时建议通过PubMed或Google Scholar检索近年研究(如2015年后)以获取更更新的实验方案和抗体验证数据。
The ERK1/2 (Phospho-Thr202/Tyr204) antibody is a widely used tool in molecular biology to detect the activated (phosphorylated) forms of extracellular signal-regulated kinases 1 and 2 (ERK1/2), key components of the MAPK signaling pathway. ERK1/2 are serine/threonine kinases that regulate diverse cellular processes, including proliferation, differentiation, survival, and apoptosis. Their activation occurs through dual phosphorylation at conserved threonine (Thr202 in ERK1/Thr185 in ERK2) and tyrosine (Tyr204 in ERK1/Tyr187 in ERK2) residues within the activation loop, typically triggered by growth factors, cytokines, or stress signals via the Ras-Raf-MEK-ERK cascade.
This phospho-specific antibody recognizes ERK1/2 only when phosphorylated at these residues, enabling researchers to distinguish active from inactive ERK1/2. It is commonly employed in techniques like Western blotting, immunofluorescence, and flow cytometry to study MAPK pathway activation in response to stimuli or inhibitors. Applications range from cancer research (e.g., assessing oncogenic signaling) to drug discovery (e.g., evaluating kinase inhibitor efficacy). Validation often includes testing in stimulated vs. unstimulated cells or tissues, with specificity confirmed using phosphorylation-blocking peptides or siRNA knockdown. Proper controls are essential, as cross-reactivity with other phosphorylated kinases or nonspecific binding can occur. Its utility in both basic research and translational studies underscores its importance in understanding cellular signaling dynamics.
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