| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KLS, SRK, S6K2, STK14B, p70S6Kb |
| Entrez GeneID | 6199; |
| WB Predicted band size | 53kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 423 (P-V-S(p)-P-L) derived from Human p70 S6 Kinase beta. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是与p70S6 Kinase beta (Phospho-Ser423)抗体相关的3篇参考文献的简要总结(注：实际文献中可能使用不同命名方式，需结合具体研究验证)：
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1. **文献名称**: *"Regulation of mTOR-dependent phosphorylation of Ser423 in p70 S6 kinase by amino acids"*
**作者**: Wang X 等
**摘要**: 研究揭示了氨基酸通过mTORC1信号调控p70S6K beta亚型Ser423位点的磷酸化，并利用特异性抗体证明该修饰对激酶活性和细胞增殖的影响。
2. **文献名称**: *"Phosphorylation of p70S6K beta at Ser423 correlates with cell size in cancer models"*
**作者**: Lee CH 等
**摘要**: 通过Phospho-Ser423抗体检测发现，该位点的磷酸化水平与肿瘤细胞体积增大相关，提示其在mTOR通路介导的细胞生长调控中的关键作用。
3. **文献名称**: *"Antibody-specific detection of p70S6K phosphorylation states in insulin signaling"*
**作者**: Magnuson B 等
**摘要**: 开发并验证了针对p70S6K beta Ser423磷酸化位点的特异性抗体，证实其在胰岛素刺激下通过PI3K/Akt通路被激活，可用于Western blot和免疫荧光分析。
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**注意**：以上为模拟简化示例，实际文献需通过PubMed或SciHub等平台检索关键词(如“p70S6K Ser423 phosphorylation antibody”)获取。若研究较少，可扩展至p70S6K其他磷酸化位点(如Thr389/Ser424)或亚型(如p70S6K alpha)。
The p70 S6 Kinase beta (p70S6Kβ), a member of the AGC kinase family, plays a critical role in the mTOR (mechanistic target of rapamycin) signaling pathway, regulating cell growth, proliferation, and protein synthesis via phosphorylation of the ribosomal protein S6. The phosphorylation of Ser423. located in the hydrophobic motif of p70S6Kβ, is a key regulatory event required for its full activation. This post-translational modification is mediated by the mTOR complex 1 (mTORC1) and facilitates kinase conformational changes, enabling substrate recognition and catalytic activity.
Antibodies specific for p70S6Kβ phosphorylated at Ser423 (Phospho-Ser423) are essential tools for studying mTOR pathway activation and its dysregulation in diseases such as cancer, diabetes, and immune disorders. These antibodies enable detection of the active, phosphorylated form of p70S6Kβ in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers utilize them to investigate cellular responses to growth factors, nutrient availability, or stress, as well as to evaluate therapeutic interventions targeting mTOR signaling. Validation of these antibodies typically includes testing in knockout models or phosphatase-treated samples to confirm specificity. Understanding p70S6Kβ activation via Ser423 phosphorylation provides insights into metabolic reprogramming and abnormal cell growth mechanisms, making this antibody valuable in both basic research and drug development.
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