| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STX1, HPC-1, P35-1, SYN1A |
| Entrez GeneID | 6804; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 14 (K-D-S(p)-D-D) derived from Human Syntaxin 1A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Syntaxin 1A (Phospho-Ser14)抗体的3篇参考文献示例，涵盖抗体的应用及功能研究：
1. **文献名称**：*"Phosphorylation of Syntaxin 1A by Protein Kinase C Modulates Synaptic Vesicle Exocytosis"*
**作者**：Fujita Y. et al.
**摘要**：研究证明Syntaxin 1A的Ser14位点磷酸化通过PKC调控突触囊泡与质膜的融合，抗体用于检测磷酸化水平变化及其对神经递质释放的影响。
2. **文献名称**：*"Site-specific Phosphorylation of Syntaxin 1A Alters Presynaptic Plasticity in Hippocampal Neurons"*
**作者**：Burkhardt P. et al.
**摘要**：利用Phospho-Ser14抗体发现，Ser14磷酸化通过影响SNARE复合体稳定性调节短期突触可塑性，并在海马神经元中验证其功能。
3. **文献名称**：*"Development and Validation of a Phosphospecific Antibody for Syntaxin 1A (Ser14) in Neuroendocrine Cells"*
**作者**：Lang T. et al.
**摘要**：报道了Phospho-Ser14抗体的开发与验证，证明其在神经内分泌细胞中特异性识别磷酸化Syntaxin 1A，并用于研究分泌囊泡动态调控机制。
(注：以上文献为示例，实际引用时需根据具体研究补充真实发表信息。)
Syntaxin 1A (Phospho-Ser14) antibody is a specialized tool used to study the phosphorylation state of Syntaxin 1A, a key SNARE protein involved in vesicle trafficking and neurotransmitter release. Syntaxin 1A is predominantly localized to presynaptic membranes, where it mediates the fusion of synaptic vesicles with the plasma membrane during exocytosis. Phosphorylation at Serine 14 (Ser14) is a post-translational modification that modulates Syntaxin 1A's interaction with other SNARE proteins (e.g., SNAP-25 and VAMP) and regulatory factors like Munc18-1. influencing vesicle docking, priming, and release dynamics.
This antibody specifically recognizes Syntaxin 1A when phosphorylated at Ser14. enabling researchers to investigate the regulatory role of this modification in cellular processes. Studies suggest that Ser14 phosphorylation may alter Syntaxin 1A's conformation, affecting its binding capacity and ultimately synaptic transmission efficiency. It has been implicated in neuroendocrine secretion, synaptic plasticity, and pathologies such as neurodegenerative disorders, diabetes, and cancer. The antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation status in brain tissues, neuronal cell lines, or secretory cells under varying physiological or experimental conditions (e.g., kinase/phosphatase modulation). Validation often includes phosphatase-treated controls or site-directed mutagenesis to confirm specificity. Its application aids in unraveling molecular mechanisms underlying exocytosis and related therapeutic interventions.
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