Rabbit Polyclonal GATA4(Phospho-Ser105) Antibody

The GATA4 (Phospho-Ser105) antibody is a specialized tool used to detect the phosphorylated form of GATA4 at serine residue 105. a post-translational modification critical for regulating its transcriptional activity. GATA4 is a zinc-finger transcription factor essential for embryonic development, cardiac morphogenesis, and tissue-specific gene expression. Phosphorylation at Ser105. mediated by kinases such as protein kinase A (PKA) or extracellular signal-regulated kinase (ERK), enhances GATA4’s DNA-binding affinity, nuclear localization, and interaction with co-factors like NKX2-5. thereby modulating its role in cell differentiation, survival, and stress responses. This modification is implicated in pathological conditions, including cardiac hypertrophy, heart failure, and certain cancers. The antibody enables researchers to study the activation status of GATA4 in signaling pathways and disease models, particularly in cardiovascular research. It is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence, often requiring validation via phosphorylation-specific controls (e.g., phosphatase treatment). Proper sample preparation with phosphatase inhibitors is crucial to preserve the phosphorylation state. Commercial variants are typically validated for specificity and sensitivity across human, mouse, and rat samples.