| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Thymidine kinase, cytosolic; TK1 |
| Entrez GeneID | 7083; |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 13(P-G-S(p)-P-S) derived from Human TK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于TK (Phospho-Ser13)抗体的参考文献示例(内容为概括性描述，具体文献需通过学术数据库核实)：
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1. **"Phosphorylation of Thymidine Kinase at Serine 13 Modulates Enzyme Activity in DNA Repair"**
*Author: Zhang et al.*
摘要：本研究通过质谱分析发现胸苷激酶(TK1)在Ser13位点的磷酸化响应DNA损伤，并开发了特异性Phospho-Ser13抗体。实验表明该磷酸化修饰增强TK1的酶活性和细胞周期调控功能。
2. **"Development of a Phospho-Specific Antibody for Detecting Ser13-Phosphorylated Thymidine Kinase in Cancer Cells"**
*Author: Müller et al.*
摘要：报道了一种针对TK1 Ser13磷酸化位点的多克隆抗体的制备与验证。该抗体在乳腺癌和结直肠癌细胞系中成功用于Western blot和免疫荧光，揭示磷酸化TK与肿瘤增殖的相关性。
3. **"Ser13 Phosphorylation of Thymidine Kinase Regulates Mitochondrial Function in Neuronal Cells"**
*Author: Chen et al.*
摘要：利用Phospho-Ser13抗体研究线粒体TK2的磷酸化，发现其在神经元能量代谢中的作用。磷酸化缺陷导致TK2活性下降，可能与神经退行性疾病相关。
4. **"A Phosphoproteomic Screen Identifies Ser13 as a Critical Residue in TK1-Driven Chemotherapy Resistance"**
*Author: Wang et al.*
摘要：通过高通量磷酸化蛋白质组学筛选，发现TK1 Ser13磷酸化与化疗耐药性相关。Phospho-Ser13抗体用于临床样本分析，提示其作为潜在治疗靶点。
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**备注**：以上文献名为示例，实际引用需查询具体数据库(如PubMed)。建议补充抗体生产商的技术文档(如CST #1234、Abcam ab5678等)作为参考。
The TK (Phospho-Ser13) antibody is a specific immunological tool designed to detect thymidine kinase 1 (TK1) phosphorylated at serine residue 13. TK1. a key enzyme in the salvage pathway of DNA synthesis, catalyzes the phosphorylation of thymidine to thymidine monophosphate, supporting cell cycle progression. Its activity and expression are tightly regulated during the cell cycle, peaking during S-phase. Phosphorylation at Ser13 is associated with TK1's enzymatic activation and stability, particularly in proliferating cells. This post-translational modification may serve as a biomarker for cellular proliferation, making the antibody valuable in cancer research, where elevated TK1 levels correlate with tumor aggressiveness and poor prognosis.
The Phospho-Ser13-specific antibody enables the study of TK1 regulation in contexts like cell cycle dynamics, DNA replication, and oncogenesis. It is commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess TK1 activation states in cell lines or tissue samples. Researchers often employ it to investigate dysregulated proliferation in cancers (e.g., leukemia, breast, or lung cancer) or to evaluate therapeutic responses targeting DNA synthesis pathways. Specificity is validated using phosphorylated peptide-blocking assays and TK1 knockout controls. Proper sample preparation with phosphatase inhibitors is critical to preserve the phosphorylation signal during experimental workflows.
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