| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | S6K, PS6K, S6K1, STK14A, p70-S6K |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 229 (N-H-Q(p)-G-H) derived from Human p70 S6 Kinase. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p70S6 Kinase (Phospho-Thr229)抗体的参考文献示例(注：文献为虚构示例，仅作格式参考)：
1. **"Regulation of p70S6 Kinase Activity by Thr229 Phosphorylation in mTOR Signaling"**
*Authors: Lee, J. et al.*
摘要：研究揭示了mTORC1通过磷酸化p70S6K的Thr229位点调控其激酶活性，并利用Phospho-Thr229抗体在细胞系中验证了该修饰对核糖体蛋白S6磷酸化的促进作用。
2. **"Phospho-Thr229 p70S6K Antibody Application in Cancer Cell Proliferation Assays"**
*Authors: Zhang, Y. et al.*
摘要：通过Western blot和免疫荧光技术，该研究使用Phospho-Thr229特异性抗体证明肿瘤细胞中p70S6K的异常激活与化疗耐药性相关，为靶向治疗提供依据。
3. **"Development and Validation of a Phospho-Specific Antibody for p70S6K Thr229"**
*Authors: Thompson, R. et al.*
摘要：报道了一种高特异性Phospho-Thr229抗体的开发，通过肽段免疫和表位验证，证实其在体外和体内实验中检测mTOR通路活性的可靠性。
4. **"Role of p70S6K Phosphorylation in Metabolic Disease Models"**
*Authors: Gupta, S. et al.*
摘要：利用Phospho-Thr229抗体分析小鼠肝脏组织，发现高脂饮食诱导的胰岛素抵抗与p70S6K Thr229磷酸化水平升高显著相关。
(提示：实际文献需通过PubMed/Google Scholar等平台检索关键词“p70S6K Thr229 phosphorylation antibody”获取。)
The p70 S6 Kinase (Phospho-Thr229) antibody is a crucial tool for studying the mTOR (mammalian target of rapamycin) signaling pathway, which regulates cell growth, proliferation, and survival. p70 S6 kinase (p70S6K), a serine/threonine kinase, exists in two isoforms (p70S6K1 and p70S6K2) and is activated via phosphorylation at multiple sites. Phosphorylation at Thr229. located in the catalytic domain, is essential for kinase activity and occurs through PDK1 (phosphoinositide-dependent kinase-1) following mTORC1-mediated phosphorylation at Thr389. This post-translational modification serves as a biomarker for mTORC1 pathway activation.
The p7S6 Kinase (Phospho-Thr229) antibody specifically detects p70S6K when phosphorylated at Thr229. enabling researchers to assess its activation status in response to growth factors, nutrients, or stress. It is widely used in Western blotting, immunofluorescence, and immunoprecipitation to investigate signaling dynamics in diseases like cancer, diabetes, and immune disorders, where mTOR pathway dysregulation is implicated. Validation typically includes testing in knockout cells or with phosphorylation-blocking reagents to confirm specificity. By monitoring Thr229 phosphorylation, this antibody helps elucidate therapeutic responses to mTOR inhibitors and contributes to mechanistic studies of cellular metabolism and protein synthesis via ribosomal protein S6 phosphorylation downstream.
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