Rabbit Polyclonal Fyn(Phospho-Tyr530) Antibody

中文名： Fyn (Phospho-Tyr530)抗体
别名： SLK, SYN, p59-FYN

货号: IPDX40637

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	SLK, SYN, p59-FYN
Entrez GeneID	2534;
WB Predicted band size	59kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of Tyrosine 530 (P-Q-Y(p)-Q-P) derived from Human Fyn.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of extracts from Mouse brain tissue using Fyn (Phospho-Tyr530) Antibody P40637.The lane on the left is treated with the antigen-specific peptide.

参考文献

以下是3篇与Fyn (Phospho-Tyr530)抗体相关的参考文献摘要(基于公开研究框架整理，非实时数据)：

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1. **文献名称**: *"Regulation of Fyn kinase activity by C-terminal phosphorylation"*

**作者**: Cheng J. et al. (2015)

**摘要**: 研究揭示了Fyn激酶通过C端Tyr530磷酸化的自我抑制机制，使用Phospho-Tyr530特异性抗体证明该位点磷酸化抑制激酶活性，并影响其在神经元突触中的信号传导功能。

2. **文献名称**: *"Role of Fyn in T-cell receptor signaling through phosphorylation of Lck"*

**作者**: Palacios E.H. & Weiss A. (2004)

**摘要**: 通过Phospho-Tyr530抗体检测发现，T细胞激活过程中Fyn的Tyr530去磷酸化促进其与Lck激酶的相互作用，调控下游ZAP-70信号通路，揭示其在免疫应答中的关键作用。

3. **文献名称**: *"Aberrant Fyn phosphorylation promotes glioblastoma progression"*

**作者**: Zhang Y. et al. (2019)

**摘要**: 利用Phospho-Tyr530抗体检测胶质母细胞瘤样本，发现Tyr530低磷酸化导致Fyn持续激活，促进肿瘤细胞侵袭和STAT3信号通路异常，为靶向治疗提供依据。

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**注意**：以上文献信息为模拟示例，实际引用需通过PubMed、Google Scholar等平台核实具体作者、标题及摘要内容。建议使用关键词 **"Fyn Tyr530 phosphorylation antibody"** 或 **"Fyn phospho-Y530"** 检索最新研究。

背景信息

The Fyn (Phospho-Tyr530) antibody is a specialized tool used to detect the phosphorylated form of Fyn kinase at tyrosine residue 530 (Tyr530). Fyn, a member of the Src family of non-receptor tyrosine kinases (SFKs), plays critical roles in signal transduction pathways regulating cell proliferation, differentiation, adhesion, and survival. Its activity is tightly controlled by phosphorylation at two key regulatory sites: Tyr530 (inhibitory) and Tyr419 (activating). Phosphorylation at Tyr530 induces a closed, inactive conformation by stabilizing intramolecular interactions between the SH2 domain and phosphorylated Tyr530. as well as the SH3 domain and a proline-rich linker. This inhibitory modification is primarily mediated by C-terminal Src kinase (Csk). Conversely, dephosphorylation of Tyr530 by phosphatases like PTPα activates Fyn, enabling its participation in downstream signaling.

The Fyn (Phospho-Tyr530) antibody is essential for studying Fyn’s regulatory mechanisms, particularly in contexts such as cancer, immune responses, and neurological disorders where dysregulated SFK activity is implicated. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess Fyn activation status in cellular models or tissue samples. It helps elucidate how extracellular stimuli (e.g., growth factors, integrin engagement) or pathological mutations modulate Fyn activity. Specificity validation, including knockout controls and phosphatase treatment, is critical to ensure accurate detection. This antibody is a key reagent for exploring Fyn’s role in diseases and its potential as a therapeutic target.