Rabbit Polyclonal Dynamin-1(Phospho-Ser774) Antibody

The Dynamin-1 (Phospho-Ser774) antibody is a specialized tool used to study the phosphorylation state of Dynamin-1. a large GTPase critical for membrane fission during clathrin-mediated endocytosis, particularly in neuronal synaptic vesicle recycling. Dynamin-1 activity is tightly regulated by post-translational modifications, including phosphorylation at Serine 774 (Ser774), a key residue located within its proline-rich domain (PRD). Phosphorylation at Ser774 is mediated by cyclin-dependent kinase 5 (Cdk5) and has been implicated in modulating Dynamin-1’s interactions with accessory proteins like endophilin and amphiphysin, which are essential for membrane scission. This modification is thought to fine-tune synaptic transmission by regulating the efficiency of vesicle retrieval under high neuronal activity. The antibody specifically recognizes Dynamin-1 when phosphorylated at Ser774. enabling researchers to investigate activity-dependent changes in synaptic vesicle dynamics, neuronal signaling, and related pathologies (e.g., neurological disorders linked to defective endocytosis). It is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation levels in cell or tissue samples. Proper validation, including knockout controls or phosphatase treatment, is essential to confirm specificity due to potential cross-reactivity with homologous phosphorylation sites in related proteins. This antibody serves as a critical reagent for exploring Dynamin-1’s regulatory mechanisms in both physiological and disease contexts.