Rabbit Polyclonal c-Abl(Phospho-Tyr245) Antibody

The c-Abl (Phospho-Tyr245) antibody detects the activated form of the c-Abl tyrosine kinase, a non-receptor protein encoded by the *ABL1* gene. c-Abl plays critical roles in cell proliferation, differentiation, adhesion, and stress response, with its activity tightly regulated by intramolecular interactions. Phosphorylation at Tyr245. located in the linker region between the SH2 and kinase domains, disrupts autoinhibitory conformations, enhancing kinase activity. This post-translational modification is associated with c-Abl activation in response to DNA damage, oxidative stress, or growth factor signaling. Dysregulation of c-Abl, particularly through constitutive activation (e.g., BCR-ABL fusion in chronic myeloid leukemia), is linked to oncogenesis. The c-Abl (Phospho-Tyr245) antibody is widely used in research to study c-Abl activation dynamics, signaling pathways (e.g., apoptosis, cytoskeletal reorganization), and therapeutic responses in cancers or neurodegenerative disorders. It is validated for applications like Western blotting, immunoprecipitation, and immunofluorescence, aiding in mechanistic studies of tyrosine kinase inhibitors (e.g., imatinib) or DNA damage response pathways. Specificity is confirmed using knockout controls or phosphorylation-blocking peptides.