Rabbit Polyclonal AurB(Phospho-Thr232) Antibody

Aurora kinase B (AurB), a member of the serine/threonine kinase family, plays a critical role in regulating mitotic progression, including chromosome alignment, spindle assembly, and cytokinesis. The Phospho-Thr232 (pThr232) epitope specifically marks the autophosphorylation site within the Aurora B activation loop, a post-translational modification essential for its full catalytic activity. This phosphorylation event is tightly linked to Aurora B’s function in the chromosomal passenger complex (CPC), where it ensures proper kinetochore-microtubule attachments and activation of the spindle assembly checkpoint. Antibodies targeting AurB (Phospho-Thr232) are valuable tools for studying mitotic dynamics, as they enable detection of the activated kinase during specific cell cycle phases. Researchers frequently use these antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate Aurora B’s spatiotemporal regulation in normal and pathological contexts, such as cancer. Overexpression or dysregulation of Aurora B is associated with genomic instability and tumorigenesis, making pThr232-specific antibodies particularly relevant in cancer research and therapeutic development. These reagents help assess Aurora B activation status in response to targeted inhibitors or cellular stressors, offering insights into mitotic checkpoint mechanisms and potential anticancer strategies.