| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CALM; CALM1; CALM2; CALM3; CAM |
| Entrez GeneID | 808; |
| WB Predicted band size | 17kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine79/serine81(K-D-T(p)-D-S(p)-E-E) derived from Human Calmodulin . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Calmodulin (Phospho-Thr79/Ser81)抗体的3篇参考文献的简要概括(注：因具体文献数据库访问限制，以下为模拟示例)：
1. **文献名称**：*Phosphorylation of Calmodulin at Thr79/Ser81 Modulates Calcium-Dependent Signaling in Neuronal Cells*
**作者**：Li, X. et al.
**摘要**：研究了Calmodulin在Thr79/Ser81位点的磷酸化对其与钙离子结合能力的影响，利用特异性抗体通过Western blot和免疫荧光验证了磷酸化水平在神经元活动中的动态变化。
2. **文献名称**：*A Novel Role of Calmodulin Phosphorylation in Cardiac Hypertrophy*
**作者**：Wang, Y. et al.
**摘要**：探讨了心肌细胞中Calmodulin Thr79/Ser81磷酸化在病理性心脏肥大中的作用，使用该抗体证明磷酸化水平与钙调蛋白依赖性激酶(CaMKII)活性增强相关。
3. **文献名称**：*Development and Validation of a Phospho-Specific Antibody for Calmodulin Thr79/Ser81*
**作者**：Smith, J. & Patel, R.
**摘要**：描述了针对Calmodulin Thr79/Ser81磷酸化表位的抗体制备与验证，包括抗体在ELISA、免疫沉淀中的特异性测试及其在细胞应激模型中的应用。
**备注**：实际文献需通过PubMed或抗体供应商(如CST的#12786抗体)的引用列表获取，部分研究可能聚焦于特定疾病模型或信号通路。若文献不足，建议补充关键词(如“CaM phosphorylation”)扩大检索范围。
Calmodulin (CaM) is a highly conserved calcium-binding protein that plays a central role in calcium-mediated signaling pathways. It regulates diverse cellular processes, including enzyme activity, ion transport, and cytoskeletal organization, by interacting with target proteins in a Ca²⁺-dependent manner. Post-translational modifications, such as phosphorylation, fine-tune its functional diversity. Phosphorylation at Thr79 and Ser81 residues, located in the flexible central helix of CaM, has been implicated in modulating its interactions with binding partners and altering downstream signaling. These phosphorylation events are thought to occur in response to specific stimuli, potentially mediated by kinases such as Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), and may influence cellular processes like neurotransmitter release, apoptosis, or cell cycle progression.
The Calmodulin (Phospho-Thr79/Ser81) antibody is a specialized tool designed to detect CaM phosphorylated at these specific residues. It enables researchers to study the spatiotemporal dynamics of CaM phosphorylation under physiological or pathological conditions, such as during neuronal activation, cardiac stress, or cancer progression. This antibody is commonly validated using techniques like western blotting, immunofluorescence, or immunoprecipitation, often with phosphorylation-specific controls (e.g., λ-phosphatase treatment or kinase inhibitors) to confirm specificity. Its applications extend to exploring how site-specific CaM phosphorylation contributes to disease mechanisms or adaptive cellular responses, offering insights into calcium signaling complexity beyond canonical activation pathways.
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