Rabbit Polyclonal IKK-gamma(Phospho-Ser31) Antibody

The IKK-gamma (Phospho-Ser31) antibody is designed to detect the phosphorylation of the IKK-gamma subunit (also known as NEMO, NF-κB essential modulator) at serine residue 31. IKK-gamma is a critical regulatory component of the IκB kinase (IKK) complex, which governs the activation of NF-κB transcription factors. This complex, composed of catalytic subunits IKK-alpha and IKK-beta along with regulatory IKK-gamma, phosphorylates IκB proteins, leading to their degradation and subsequent nuclear translocation of NF-κB to mediate immune, inflammatory, and stress responses. Phosphorylation at Ser31 is implicated in regulating the activity or stability of IKK-gamma, particularly in response to stimuli like cytokines (e.g., TNF-α, IL-1), pathogens, or DNA damage. This post-translational modification may influence interactions with upstream signaling molecules or modulate the overall function of the IKK complex. The IKK-gamma (Phospho-Ser31) antibody is widely used in research to investigate NF-κB pathway activation mechanisms, particularly in contexts such as inflammation, autoimmune disorders, and cancer. Its specificity for the phosphorylated form allows researchers to study dynamic regulatory events in signaling cascades. Proper validation, including knockout controls or peptide competition assays, is essential to confirm antibody specificity, and sample preparation must preserve phosphorylation states using phosphatase inhibitors.