| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADRB2R; B2AR; adrenergic receptor; beta-2; |
| Entrez GeneID | 154; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 346 (R-S-S(p)-L-K) derived from Human Adrenergic Receptor beta2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Adrenergic Receptor beta2 (Phospho-Ser346)抗体的参考文献及摘要概括：
1. **文献名称**：*"Phosphorylation of the β2-Adrenergic Receptor in Airway Smooth Muscle"*
**作者**：Penn RB et al.
**摘要**：研究揭示了β2-AR在Ser346位点的磷酸化通过G蛋白偶联受体激酶(GRK)介导，参与受体脱敏和细胞内吞，影响支气管扩张功能。
2. **文献名称**：*"Regulation of β2-Adrenergic Receptor Signaling by Phosphorylation of Ser346"*
**作者**：Tilley DG, Rockman HA.
**摘要**：通过磷酸化特异性抗体(Phospho-Ser346)证实，该位点的磷酸化在心力衰竭模型中调节β2-AR与抑制性G蛋白(Gi)的偶联，影响心脏功能。
3. **文献名称**：*"Site-Specific Phosphorylation of β2-Adrenergic Receptor Modulates Its Trafficking and Signaling"*
**作者**：Moore RH et al.
**摘要**：利用Phospho-Ser346抗体发现，该位点磷酸化通过网格蛋白介导的内吞途径调控受体循环，并影响下游cAMP/PKA信号通路。
4. **文献名称**：*"β2-Adrenergic Receptor Phosphorylation at Ser346 Controls Desensitization in Asthmatic Airway Smooth Muscle"*
**作者**：Billington CK et al.
**摘要**：研究通过Phospho-Ser346抗体证明，哮喘患者气道平滑肌中该位点的异常磷酸化与β2-AR功能减退及糖皮质激素治疗反应相关。
以上文献均使用Phospho-Ser346抗体探究β2-AR磷酸化在疾病机制或信号通路中的功能。
The adrenergic receptor beta2 (ADRB2) is a G protein-coupled receptor (GPCR) that plays a central role in mediating physiological responses to catecholamines like epinephrine and norepinephrine. It is widely expressed in tissues such as the lungs, vascular smooth muscle, and skeletal muscle, regulating processes like bronchodilation, vasodilation, and metabolic activity. Phosphorylation of ADRB2 at Serine 346 (Ser346) is a key post-translational modification associated with receptor desensitization and internalization, a feedback mechanism to attenuate prolonged signaling. This phosphorylation event is primarily mediated by G protein-coupled receptor kinases (GRKs) or protein kinase A (PKA), facilitating β-arrestin binding and subsequent receptor internalization via clathrin-coated pits.
Antibodies specific to ADRB2 phosphorylated at Ser346 are critical tools for studying receptor regulation in physiological and pathological contexts. They enable researchers to detect and quantify the activated/desensitized state of ADRB2 using techniques like Western blotting, immunofluorescence, or immunoprecipitation. Such antibodies are particularly relevant in research on asthma, heart failure, and metabolic disorders, where dysregulated ADRB2 signaling contributes to disease progression. Additionally, they aid in evaluating the efficacy of beta-agonists or antagonists, as well as understanding resistance mechanisms in chronic therapies. Validated phospho-specific antibodies ensure specificity by distinguishing phosphorylated ADRB2 from other isoforms or unmodified receptors, providing insights into dynamic receptor trafficking and signaling pathways.
×
