Rabbit Polyclonal Pin1(Phospho-Ser16) Antibody

The Pin1 (Phospho-Ser16) antibody is a specialized tool used to detect the phosphorylated form of Pin1 at serine residue 16 (Ser16). Pin1. a peptidyl-prolyl cis-trans isomerase (PPIase), plays a critical role in regulating protein conformation and function by catalyzing the isomerization of phosphorylated serine/threonine-proline (pSer/Thr-Pro) motifs. This post-translational modification influences diverse cellular processes, including cell cycle progression, transcriptional regulation, and stress responses. Phosphorylation at Ser16 is implicated in modulating Pin1's activity, subcellular localization, or interactions with substrates. For instance, studies suggest that phosphorylation at this site may regulate Pin1’s nuclear-cytoplasmic shuttling or its involvement in signaling pathways such as Wnt/β-catenin or NF-κB. The Pin1 (Phospho-Ser16) antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate Pin1's phosphorylation status in physiological or pathological contexts, such as cancer, neurodegeneration, or aging. Its specificity for the phosphorylated Ser16 epitope allows researchers to dissect Pin1's regulatory mechanisms and its role in diseases where Pin1 dysregulation is observed, such as Alzheimer’s disease or breast cancer. Validating this antibody’s specificity (e.g., via knockout controls or phosphatase treatment) is essential to ensure accurate detection of this post-translationally modified form.