| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B-cell linker protein; LY57; SLP-65; SLP65; |
| Entrez GeneID | 29760; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 96(D-S-Y(p)-E-P) derived from Human BLNK . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BLNK (Phospho-Tyr96)抗体的假设性参考文献示例(内容为虚构，仅供参考)：
1. **"BLNK Tyrosine Phosphorylation Regulates B Cell Receptor Signaling"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过使用BLNK (Phospho-Tyr96)特异性抗体，揭示了Tyr96磷酸化在B细胞受体(BCR)下游信号传导中的关键作用，并证明其与Syk激酶的相互作用增强免疫突触形成。
2. **"Phosphorylation of BLNK at Tyr96 Modulates B Cell Development"**
- **作者**: Lee C, et al.
- **摘要**: 利用Phospho-Tyr96抗体进行免疫沉淀和流式细胞术分析，发现Tyr96位点磷酸化缺陷导致小鼠B细胞发育停滞，强调了该位点在V(D)J重组中的功能。
3. **"Targeting BLNK Phosphorylation in B Cell Malignancies"**
- **作者**: Garcia R, et al.
- **摘要**: 通过Western blot和免疫组化验证BLNK (Phospho-Tyr96)抗体在慢性淋巴细胞白血病(CLL)样本中的应用，发现磷酸化水平与疾病进展和药物耐药性相关。
4. **"Development and Validation of a Phospho-Specific Antibody for BLNK Tyr96"**
- **作者**: Wang Y, et al.
- **摘要**: 描述了BLNK (Phospho-Tyr96)抗体的制备及特异性验证，通过突变实验和质谱分析确认其仅识别Tyr96磷酸化形式，并应用于B细胞活化的动力学研究。
(注：以上文献为模拟生成，实际引用需查询真实数据库如PubMed。)
The BLNK (B-cell linker protein), also known as SLP-65. is a critical adaptor protein in B-cell receptor (BCR) signaling. Upon BCR activation, BLNK undergoes phosphorylation at specific tyrosine residues, including Tyr96. which serves as a docking site for downstream signaling molecules. Phosphorylation at Tyr96 is mediated by Syk kinase and facilitates the assembly of a multiprotein signaling complex involving PLCγ, BTK, and Vav. This complex triggers key pathways like calcium mobilization, NF-κB activation, and MAPK signaling, regulating B-cell development, proliferation, and differentiation.
The BLNK (Phospho-Tyr96) antibody specifically detects BLNK when phosphorylated at tyrosine 96. enabling researchers to study BCR signaling dynamics. This antibody is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess activation status in B-cell malignancies, autoimmune disorders, or immunodeficiency diseases linked to dysregulated BCR signaling. Its specificity helps elucidate molecular mechanisms in pathological contexts, including aberrant B-cell activation or defective immune responses. Validated applications often include research on lymphomas, leukemias, and conditions like agammaglobulinemia, where BLNK mutations or phosphorylation defects impair B-cell function. Proper controls (e.g., phosphorylation inhibitors) are recommended to confirm signal specificity.
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