| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DKFZp762A223; FA-D2; FA4; FACD; Fanconi anemia |
| Entrez GeneID | 2177; |
| WB Predicted band size | 166kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 222 (G-D-S(p)-Q-H) derived from Human FANCD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FANCD2 (Phospho-Ser222)抗体的参考文献，简要整理如下：
1. **文献名称**：*Phosphorylation of FANCD2 Inhibits the FANCD2/BRCA2 Pathway in Human Cells*
**作者**：Ishiai, M. et al.
**摘要**：研究揭示了FANCD2在Ser222位点的磷酸化通过抑制其单泛素化，调控Fanconi贫血通路对DNA交联损伤的响应，实验中使用Phospho-Ser222抗体验证了磷酸化与功能失活的关联。
2. **文献名称**：*A Conserved Phosphorylation Switch Controls the Interaction between FANCD2 and BRCA2*
**作者**：Collins, N.B. et al.
**摘要**：通过Phospho-Ser222特异性抗体，研究发现DNA损伤后FANCD2的Ser222磷酸化阻碍其与BRCA2的结合，揭示了该位点调控同源重组修复的分子机制。
3. **文献名称**：*Functional Analysis of FANCD2 Phosphorylation Sites in DNA Crosslink Repair*
**作者**：Ho, G.P. et al.
**摘要**：利用Phospho-Ser222抗体进行免疫印迹和免疫荧光实验，证实Ser222磷酸化是FANCD2泛素化及修复功能的关键调控步骤，突变该位点导致修复缺陷。
4. **文献名称**：*Phosphorylation-Dependent Regulation of FANCD2 Activity in the Fanconi Anemia Pathway*
**作者**：Garcia-Higuera, I. et al.
**摘要**：研究通过Phospho-Ser222抗体发现，ATM/ATR激酶介导的Ser222磷酸化是FANCD2激活并招募至DNA损伤灶的必要条件，影响其修复功能。
这些文献均通过Phospho-Ser222抗体研究了该位点的功能，涵盖其在DNA损伤响应、蛋白互作及修复通路调控中的作用。
The FANCD2 (Phospho-Ser222) antibody is a specialized tool used to detect the phosphorylated form of the Fanconi anemia complementation group D2 (FANCD2) protein at serine residue 222. FANCD2 is a central component of the Fanconi anemia (FA) DNA repair pathway, which plays a critical role in maintaining genomic stability by resolving DNA interstrand crosslinks (ICLs) and facilitating homologous recombination repair. Phosphorylation at Ser222 is a key post-translational modification that occurs in response to DNA damage or replication stress, often preceding FANCD2's monoubiquitination on lysine 561 (a hallmark of its activation). This modification is thought to regulate FANCD2's recruitment to DNA damage sites and its interaction with other repair proteins.
The antibody is widely used in research to study the DNA damage response (DDR), particularly in contexts like cancer, bone marrow failure, and Fanconi anemia. It helps assess the activation status of the FA pathway in cells exposed to genotoxic agents (e.g., mitomycin C or ionizing radiation) via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Validations typically include knockout controls or phosphatase treatment to confirm phosphorylation-specific recognition. Its applications extend to investigating chemoresistance mechanisms and developing targeted therapies for FA-deficient cancers.
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