Rabbit Polyclonal p7S6Kinase(Phospho-Thr389/412) Antibody

The p70 S6 Kinase (p70S6K), a member of the AGC family of serine/threonine kinases, plays a critical role in the mTOR (mechanistic target of rapamycin) signaling pathway, regulating cell growth, proliferation, and survival. Phosphorylation at specific residues, including Thr389 and Thr412. is essential for its activation. Thr389 phosphorylation, located in the linker domain, is mediated by mTOR complex 1 (mTORC1) and serves as a hallmark of mTORC1 activity. Thr412 phosphorylation in the activation loop is required for full kinase activity, often facilitated by PDK1 (3-phosphoinositide-dependent protein kinase 1). Antibodies targeting p70S6K phosphorylated at Thr389/412 are widely used to assess mTOR pathway activation in research contexts, such as cancer, metabolic disorders, and immune responses. These antibodies enable detection of the activated form of p70S6K via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Their specificity helps evaluate cellular responses to growth factors, nutrients, or stress, as well as the efficacy of mTOR inhibitors (e.g., rapamycin). Dysregulation of p70S6K phosphorylation is linked to diseases like cancer, diabetes, and neurodegeneration, making these antibodies valuable tools for both mechanistic studies and therapeutic development. Proper controls (e.g., phosphatase treatment) are recommended to confirm phosphorylation-dependent signals.