Rabbit Polyclonal Chk1(Phospho-Ser286) Antibody

The Chk1 (Phospho-Ser286) antibody is a specialized tool used to detect the activated form of Checkpoint kinase 1 (Chk1), a key regulator of the DNA damage response (DDR) and cell cycle progression. Chk1 is phosphorylated at specific residues, including Ser286. in response to replication stress or DNA damage, triggering its activation. This phosphorylation event is critical for Chk1's role in stabilizing stalled replication forks, delaying cell cycle progression (via G2/M checkpoint control), and promoting DNA repair. The antibody specifically recognizes Chk1 when phosphorylated at Ser286. enabling researchers to study its activation dynamics in contexts like genotoxic stress, chemotherapy, or radiation exposure. It is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to assess Chk1 signaling activity in cell lines (e.g., HeLa, U2OS) or tissues. Studies utilizing this antibody have contributed to understanding cancer biology, as Chk1 dysregulation is linked to tumorigenesis and chemoresistance. Additionally, it aids in evaluating therapeutic agents targeting the DDR pathway, particularly inhibitors combining Chk1 with DNA-damaging drugs. Validation often includes testing in cells treated with hydroxyurea, ultraviolet light, or other DNA stressors to confirm phosphorylation-dependent signals.