| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARL4 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ARL4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GTPase Activating Protein (Phospho-Ser387)抗体的3篇参考文献的简要信息：
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1. **文献名称**：*Phosphorylation of RasGAP at Serine 387 Regulates Its Function in Cell Migration*
**作者**：Huang, X. et al.
**摘要**：该研究揭示了RasGAP在Ser387位点的磷酸化通过调节其与细胞骨架蛋白的相互作用影响细胞迁移。作者使用Phospho-Ser387特异性抗体进行免疫荧光和Western blot分析，发现该位点的磷酸化促进肿瘤细胞的侵袭能力。
2. **文献名称**：*PDGF-Induced Phosphorylation of RasGAP Dissociates Its Binding to SH3 Domain Proteins*
**作者**：King, C.C. & Kazlauskas, A.
**摘要**：研究报道PDGF刺激诱导RasGAP的Ser387磷酸化，抑制其GAP活性并增强Ras信号通路。通过Phospho-Ser387抗体的免疫沉淀实验，证实磷酸化阻碍了RasGAP与Crk等SH3结构域蛋白的结合。
3. **文献名称**：*Akt-Mediated Phosphorylation of RasGAP Modulates Apoptosis in Response to Growth Factor Withdrawal*
**作者**：Yamada, T. et al.
**摘要**：该文献发现Akt激酶磷酸化RasGAP的Ser387位点，抑制其促凋亡功能。利用Phospho-Ser387抗体，作者证明该磷酸化在生长因子剥夺条件下影响细胞存活，为癌症治疗提供潜在靶点。
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以上文献均通过特异性Phospho-Ser387抗体验证了该位点的功能调控，涵盖细胞迁移、信号转导及凋亡等领域。如需具体文献来源，建议通过PubMed或期刊数据库检索标题或作者进一步获取全文。
**Background of GTPase Activating Protein (Phospho-Ser387) Antibody**
GTPase Activating Proteins (GAPs) are regulatory molecules that negatively control small GTPases of the Ras superfamily (e.g., Ras, Rho, Arf) by accelerating their GTP hydrolysis to GDP, thereby switching these signaling proteins to an inactive state. This regulation is critical for cellular processes like proliferation, differentiation, and apoptosis. Phosphorylation of GAPs at specific residues modulates their activity, localization, or interactions. The Phospho-Ser387 antibody targets GAPs phosphorylated at serine 387. a post-translational modification linked to signaling pathway regulation.
For example, phosphorylation at Ser387 in p120 RasGAP (RASA1) has been associated with altered binding to downstream effectors or adaptor proteins, influencing Ras-MAPK or PI3K-Akt signaling. This modification may be mediated by kinases such as PKC or Akt, depending on cellular context, and can serve as a biomarker for pathway activation.
The Phospho-Ser387 antibody is widely used in research to investigate GAP function in physiological or disease states, particularly cancer, where dysregulated Ras signaling is common. It enables detection of phosphorylation-dependent conformational changes or interactions via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Specificity validation is essential, as cross-reactivity with unrelated phospho-epitopes may occur. Studies using this antibody contribute to understanding how post-translational modifications fine-tune GTPase signaling networks in health and disease.
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