| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor |
| Entrez GeneID | 3486; |
| WB Predicted band size | 30kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 183(K-D-S(p)-Q-R) derived from Human IGFBP-3 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **IGFBP-3 (Phospho-Ser183)** 抗体的参考文献示例(内容为模拟，仅供参考)：
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1. **文献名称**: *"Phosphorylation of IGFBP-3 at Ser183 modulates its apoptotic function in prostate cancer cells"*
**作者**: Jones, A.B. et al.
**摘要**: 研究揭示了IGFBP-3在Ser183位点的磷酸化通过调控其与胰岛素样生长因子(IGF)的结合能力，增强前列腺癌细胞凋亡的分子机制，并使用Phospho-Ser183抗体验证了磷酸化状态对功能的影响。
2. **文献名称**: *"Role of IGFBP-3 Ser183 phosphorylation in cellular senescence and tumor suppression"*
**作者**: Lee, C. & Smith, R.K.
**摘要**: 通过免疫印迹和免疫荧光技术，证明IGFBP-3 Ser183磷酸化通过激活p53通路促进细胞衰老，Phospho-Ser183抗体被用于检测乳腺癌模型中的磷酸化水平变化。
3. **文献名称**: *"Kinase-dependent regulation of IGFBP-3 phosphorylation at Ser183 and its implications in lung cancer metastasis"*
**作者**: Zhang, H. et al.
**摘要**: 研究发现Akt激酶介导的IGFBP-3 Ser183磷酸化抑制其抗转移功能，Phospho-Ser183抗体用于临床样本分析，显示磷酸化水平与肺癌患者预后负相关。
4. **文献名称**: *"Development and validation of a phospho-specific antibody for IGFBP-3 at Ser183: Applications in metabolic studies"*
**作者**: Brown, T.P. et al.
**摘要**: 描述了Phospho-Ser183抗体的制备与特异性验证，证明其在血清样本和细胞裂解液中的检测灵敏度，并应用于2型糖尿病模型中IGFBP-3磷酸化的动态分析。
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建议通过 **PubMed** 或 **Google Scholar** 以关键词“IGFBP-3 Phospho-Ser183 antibody”或“IGFBP-3 Ser183 phosphorylation”检索最新文献，获取具体研究细节。
The IGFBP-3 (Phospho-Ser183) antibody is a specialized tool used to detect insulin-like growth factor-binding protein 3 (IGFBP-3) when phosphorylated at serine residue 183. IGFBP-3 is a key regulator of insulin-like growth factors (IGFs), particularly IGF-1 and IGF-2. modulating their bioavailability and activity in growth, metabolism, and apoptosis. Phosphorylation at Ser183 is a post-translational modification implicated in altering IGFBP-3's affinity for IGFs, potentially influencing its role in cell proliferation, survival, or nuclear signaling pathways independent of IGFs.
This antibody is widely employed in research to investigate IGFBP-3's phosphorylation-dependent functions in physiological and pathological contexts. Studies suggest phosphorylated IGFBP-3 may act as a biomarker in cancers (e.g., breast, prostate) or metabolic disorders, as its phosphorylation status correlates with disease progression or therapeutic response. It is also used to explore IGFBP-3's IGF-independent roles, such as transcriptional regulation or interactions with nuclear receptors.
Validation typically involves Western blotting, immunofluorescence, or immunohistochemistry in cell/tissue samples. Researchers must confirm specificity using phosphorylation-blocking peptides or knockout controls. Understanding Ser183 phosphorylation dynamics helps clarify IGFBP-3's dual roles in growth inhibition and promotion, offering insights into targeted therapies for IGF-axis-related diseases.
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